Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus.

Total FLC transcript dynamics from divergent paralogue expression explains flowering diversity in Brassica napus.
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DOI:
10.1111/nph.17131
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发表时间:
2021-03
期刊:
The New phytologist
影响因子:
--
通讯作者:
Morris RJ
Morris RJ
中科院分区:
其他
文献类型:
--
作者:
Calderwood A;Lloyd A;Hepworth J;Tudor EH;Jones DM;Woodhouse S;Bilham L;Chinoy C;Williams K;Corke F;Doonan JH;Ostergaard L;Irwin JA;Wells R;Morris RJ

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开花时间是一个重要的适应性和农艺性状。在拟南芥中,花抑制基因FLOWERLOCUS C(FLC)表达水平的自然变化导致春化作用的差异。在甘蓝型油菜中,FLC有9个拷贝。在这里,我们研究这些多个FLC旁系如何决定春化要求作为一个系统。我们收集了甘蓝型油菜春季、冬季、半冬季和西伯利亚羽衣甘蓝作物类型的转录组时间序列。建模被用来链接FLC表达动态花春化后的反应。我们表明,放宽选择压力,允许表达的FLC旁系分化,导致在冷处理过程中的FLC表达的变化之间的旁系和加入。我们发现,总FLC表达动态最好地解释了品种之间的冷需求的差异,而不是特定的FLC旁系同源物的表达。多个FLC旁系同源物与不同的表达动力学的组合导致丰富的行为,以应对寒冷和广泛的春化要求在B。油菜。我们发现不同的策略,以确定现有的冬油菜品种对寒冷的反应的证据。
Flowering time is a key adaptive and agronomic trait. In Arabidopsis, natural variation in expression levels of the floral repressor FLOWERING LOCUS C (FLC) leads to differences in vernalization. In Brassica napus there are nine copies of FLC. Here, we study how these multiple FLC paralogues determine vernalization requirement as a system. We collected transcriptome time series for Brassica napus spring, winter, semi‐winter, and Siberian kale crop types. Modelling was used to link FLC expression dynamics to floral response following vernalization. We show that relaxed selection pressure has allowed expression of FLC paralogues to diverge, resulting in variation of FLC expression during cold treatment between paralogues and accessions. We find that total FLC expression dynamics best explains differences in cold requirement between cultivars, rather than expression of specific FLC paralogues. The combination of multiple FLC paralogues with different expression dynamics leads to rich behaviour in response to cold and a wide range of vernalization requirements in B. napus. We find evidence for different strategies to determine the response to cold in existing winter rapeseed accessions.
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