Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration.
Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration.
复制标题
DOI：
10.1016/j.ydbio.2012.02.031
复制
发表时间：
2012-05-15
影响因子：
2.7
通讯作者：
Stankunas K
中科院分区：
文献类型：
--
作者：
Stewart S;Stankunas K
与人类不同，一些脊椎动物能够完全再生受损的肢体和其他器官。例如，成年斑马鱼会再生一个被切断的尾鳍的复杂结构，以至于原始鳍和替换鳍无法区分。胚基是再生动物肢体切除后形成的一团独特的细胞，是再生组织的主要来源。然而，对芽基有贡献的细胞谱系及其对再生鳍的最终贡献尚未明确表征。有人认为，截肢部位附近的细胞去分化形成多能祖细胞，这些祖细胞填充芽基，然后产生再生鳍的多种细胞类型。其他研究提出，芽基细胞是不均匀的群体，其贡献于不同细胞谱系的潜力仍然受到限制。我们测试了这些模型，使用诱导Cre-lox技术，以产生成年斑马鱼与不同的，孤立的群体内的尾鳍的遗传标记的细胞。然后，我们在个体动物的鳍再生的整个过程中跟踪了几种细胞类型的群体。我们没有发现多能祖细胞存在的证据。相反，多种细胞类型，包括表皮细胞，射线内成纤维细胞和成骨细胞，有助于新再生的组织，同时保持高度限制其发育身份。我们的研究进一步表明，再生鳍由许多重复的芽基“单位”专用于每个鳍射线。这些芽基都有一个有组织的结构谱系限制，去分化的细胞，合作再生尾鳍。
Unlike humans, some vertebrate animals are able to completely regenerate damaged appendages and other organs. For example, adult zebrafish will regenerate the complex structure of an amputated caudal fin to a degree that the original and replacement fins are indistinguishable. The blastema, a mass of cells that uniquely forms following appendage amputation in regenerating animals, is the major source of regenerated tissue. However, the cell lineage(s) that contribute to the blastema and their ultimate contribution(s) to the regenerated fin have not been definitively characterized. It has been suggested that cells near the amputation site dedifferentiate forming multipotent progenitors that populate the blastema and then give rise to multiple cell types of the regenerated fin. Other studies propose that blastema cells are non-uniform populations that remain restricted in their potential to contribute to different cell lineages. We tested these models by using inducible Cre-lox technology to generate adult zebrafish with distinct, isolated groups of genetically labeled cells within the caudal fin. We then tracked populations of several cell types over the entire course of fin regeneration in individual animals. We found no evidence for the existence of multipotent progenitors. Instead, multiple cell types, including epidermal cells, intra-ray fibroblasts, and osteoblasts, contribute to the newly regenerated tissue while remaining highly restricted with respect to their developmental identity. Our studies further demonstrate that the regenerating fin consists of many repeating blastema “units” dedicated to each fin ray. These blastema each have an organized structure of lineage restricted, dedifferentiated cells that cooperate to regenerate the caudal fin.
登录
查看更多内容
影响因子：
1.5
作者：
DeLaurier, April;Eames, B. Frank;Blanco-Sanchez, Bernardo;Peng, Gang;He, Xinjun;Swartz, Mary E.;Ullmann, Bonnie;Westerfield, Monte;Kimmel, Charles B.
通讯作者：
Kimmel, Charles B.
影响因子：
11.8
作者：
Knopf, Franziska;Hammond, Christina;Weidinger, Gilbert
通讯作者：
Weidinger, Gilbert
影响因子：
4.6
作者：
Gargioli, C;Slack, JMW
通讯作者：
Slack, JMW
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
64.8
作者：
通讯作者：
--