Thermoregulation of protein synthesis in Borrelia burgdorferi
Thermoregulation of protein synthesis in Borrelia burgdorferi
复制标题
伯氏疏螺旋体蛋白质合成的温度调节
DOI：
10.1128/iai.58.4.1038-1042.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
John T. Boothby
中科院分区：
文献类型：
--
作者：
R. G. Cluss;John T. Boothby
伯氏疏螺旋体是莱姆病的病原体，通过扁虱的叮咬感染人类。这种微生物至少在两种截然不同的环境中生存：节肢动物媒介和温血宿主。我们研究了Burgdorferi B31在突然的热应激下的蛋白质合成，这与从媒介传播到宿主的过程中发生的类似。在28℃、更高温度和脉冲追逐实验中合成的蛋白质用~3H标记的氨基酸标记4h，并用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和荧光法进行表征。我们命名为热应激蛋白(HSPs)的四种蛋白质(HSP-1，75千道尔顿[kDa]；HSP-2，42 kDa；HSP-3，39 kDa；HSP-4，27 kDa)的合成随着温度的升高而增加，而我们命名为热不稳定蛋白1(29.5 kDa)的蛋白质在更高的温度下合成减少。在37到40摄氏度时，主要的热应激蛋白HSP-1占总细胞蛋白的14%到18%，而在28℃时占总细胞蛋白的1%到2%。HSP-1在40℃或28℃的4小时追逐中保持稳定。在低传代中，伯氏杆菌致病株的类似HSPs的演示表明，热应激反应在伯氏杆菌菌株中可能是常见的，并可能在莱姆病中发挥作用。
Borrelia burgdorferi, the etiological agent of Lyme disease, infects humans via the bite of a tick. The microbe survives in at least two vastly different environments: an arthropod vector and a warm-blooded host. We examined protein synthesis in B. burgdorferi B31 in response to sudden heat stress, which is similar to that which occurs during the transmission from vector to host. Proteins synthesized after shifts from 28 degrees C to higher temperatures and in pulse-chase experiments were labeled with 3H-labeled amino acids for 4 h and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. The synthesis of four proteins we designated as heat stress proteins (HSPs) was increased by shifts to higher temperatures (HSP-1, 75 kilodaltons [kDa]; HSP-2, 42 kDa; HSP-3, 39 kDa; and HSP-4, 27 kDa); and the amount of one protein we designated as heat-labile protein 1 (29.5 kDa) was decreased at higher temperatures. At 37 to 40 degrees C, the major heat stress protein, HSP-1, represented 14 to 18% of the total cell protein compared with 1 to 2% of the total cell protein at 28 degrees C. HSP-1 was stable during a 4-h chase at either 40 or 28 degrees C. Demonstration of similar HSPs in low-passage, pathogenic strains of B. burgdorferi suggests that the heat stress response may be common among B. burgdorferi strains and may play a role in Lyme disease.
鼠伤寒沙门氏菌对 HeLa 细胞的附着和侵袭：甘露糖敏感和甘露糖抗性血凝活性的贡献。
DOI：
10.1099/00221287-127-2-361
发表时间：
1981
期刊：
Journal of general microbiology
影响因子：
--
作者：
Jones,GW;Richardson,LA
通讯作者：
Richardson,LA
尿路致病性大肠杆菌粘附在患有复发性尿路感染的女性的上皮细胞上。
影响因子：
7.5
作者：
Schaeffer,AJ;Jones,JM;Duncan,JL;Chmiel,JS;Plotkin,BJ;Falkowski,WS
通讯作者：
Falkowski,WS