Collagen fiber recruitment: A microstructural basis for the nonlinear response of the posterior pole of the eye to increases in intraocular pressure.
Collagen fiber recruitment: A microstructural basis for the nonlinear response of the posterior pole of the eye to increases in intraocular pressure.
复制标题
DOI：
10.1016/j.actbio.2018.03.026
复制
发表时间：
2018-05
影响因子：
9.7
通讯作者：
Sigal IA
中科院分区：
文献类型：
--
作者：
Jan NJ;Sigal IA
我们的目标是量化和表征筛板（LC）和乳头周围巩膜（PPS）的胶原纤维卷曲波度如何随眼内压（IOP）变化。将13只绵羊（绵羊）眼睛浸没并灌注固定，同时将IOP维持在0、10、15、20或50 mmHg。用偏振光显微镜（PLM）对冠状视神经乳头（ONH）切片（30 µm）进行成像，并分析胶原纤维取向和波纹度（纤维取向的SD）。在LC中，测量每个LC光束的波度。在PPS中，每只眼睛至少测量900个胶原束。使用50 mmHg IOP下的波度，我们定义了组织特异性阈值，以确定加载或募集的纤维的分数。结果发现，纤维波纹度随眼压升高而降低（P<0.001）。在各眼压下，LC内胶原纤维的波纹度和纤维募集率均小于或等于PPS（P<0.001）。在15 mmHg IOP下，LC和PPS均具有3/4的募集纤维和1/4的储备纤维。随着IOP和相关纤维募集的波度降低是ONH的基于纤维的非线性生物力学行为的实验证据。在所有IOP下，PPS具有与LC相等或更大的纤维补充分数。LC和PPS在正常IOP下具有相同的募集和储备纤维分数，表明这可能是健康眼睛的最佳募集分数。这是否延伸到人类的眼睛仍然未知。
Our goal was to quantify and characterize how the collagen fiber crimp waviness of the lamina cribrosa (LC) and peripapillary sclera (PPS) changes with intraocular pressure (IOP). Thirteen sheep (ovine) eyes were immersion and perfusion fixed while maintaining IOP at 0, 10, 15, 20, or 50 mmHg. Coronal optic nerve head (ONH) sections (30 µm) were imaged with polarized light microscopy (PLM) and analyzed for collagen fiber orientation and waviness (SD of fiber orientation). In the LC, the waviness of every LC beam was measured. In the PPS, at least 900 collagen bundles were measured per eye. Using the waviness at 50 mmHg IOP, we defined tissue-specific thresholds to determine the fraction of loaded or recruited fibers. We found that fiber waviness decreased with IOP (P<0.001). At every IOP, the waviness of the collagen fibers, and the fraction of fibers recruited in the LC were smaller or equal than those of the PPS (P<0.001). At 15 mmHg IOP, both LC and PPS had ¾ recruited fibers and ¼ left in reserve. The decreased waviness with IOP and associated fiber recruitment is experimental evidence of fiber-based nonlinear biomechanical behavior of the ONH. At all IOPs the PPS had an equal or larger fraction of fibers recruited than the LC. That both LC and PPS had the same fraction of recruited and reserve fibers at normal IOP suggests that this may be an optimal fraction of recruitment for healthy eyes. Whether this extends to human eyes remains unknown.
登录
查看更多内容
影响因子：
3.5
作者：
Fazio, Massimo A.;Grytz, Rafael;Morris, Jeffrey S.;Bruno, Luigi;Gardiner, Stuart K.;Girkin, Christopher A.;Downs, J. Crawford
通讯作者：
Downs, J. Crawford
DOI：
10.1115/1.1427698
发表时间：
2002-02-01
影响因子：
1.7
作者：
Hansen, KA;Weiss, JA;Barton, JK
通讯作者：
Barton, JK
DOI：
10.1098/rspb.1972.0019
发表时间：
1972-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DIAMANT, J;ARRIDGE, RGC;KELLER, A
通讯作者：
KELLER, A
影响因子：
1.8
作者：
Ghaffari, Masoud Selk;Shojaei, Mohammad;Khorami, Nargess
通讯作者：
Khorami, Nargess
考虑胶原原纤维卷曲形态的角膜力学模型
影响因子：
4.4
作者：
Liu, Xiaoyu;Wang, Lizhen;Fan, Yubo
通讯作者：
Fan, Yubo