The relationship between fasting-induced torpor, sleep, and wakefulness in laboratory mice.
The relationship between fasting-induced torpor, sleep, and wakefulness in laboratory mice.
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禁食引起的torpor，睡眠和实验室小鼠的清醒之间的关系。
DOI：
10.1093/sleep/zsab093
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发表时间：
2021-09-13
期刊：
影响因子：
5.6
通讯作者：
Vyazovskiy VV
中科院分区：
文献类型：
--
作者：
Huang YG;Flaherty SJ;Pothecary CA;Foster RG;Peirson SN;Vyazovskiy VV
休眠是许多哺乳动物物种用于保存能量的调节和可逆的代谢抑制状态。尽管在季节性冬眠动物中，人们已经对麻木和睡眠之间的关系进行了充分的研究，但对禁食诱导的麻木对实验室小鼠的警觉状态和大脑活动的影响却知之甚少。连续监测脑电图（EEG），肌电图（EMG）和体表体温的成年雄性C57 BL/6小鼠在连续几天的计划限制喂养。所有动物都表现出体温过低，随着禁食的进行，体温过低的程度逐渐加深，持续时间逐渐延长。EEG和EMG明显受低温的影响，尽管非快速眼动（NREM）睡眠、快速眼动（REM）睡眠和觉醒的典型电生理特征使我们能够在所有情况下进行警戒状态分类。与以前的研究一致，低温发作是从一种与NREM睡眠无法区分的状态开始的，EEG功率随着体表温度的降低而逐渐降低。在深度低温期间，快速眼动睡眠基本上被取消，我们观察到与颤抖相关的肌肉活动的强烈爆发。我们的研究突出了小鼠禁食诱导的麻木，Djungarian仓鼠的日常麻木和季节性冬眠物种的冬眠的EEG特征之间的重要相似之处。未来的研究是必要的，以澄清禁食引起的麻木对随后的睡眠的影响。
Torpor is a regulated and reversible state of metabolic suppression used by many mammalian species to conserve energy. Whereas the relationship between torpor and sleep has been well-studied in seasonal hibernators, less is known about the effects of fasting-induced torpor on states of vigilance and brain activity in laboratory mice. Continuous monitoring of electroencephalogram (EEG), electromyogram (EMG), and surface body temperature was undertaken in adult, male C57BL/6 mice over consecutive days of scheduled restricted feeding. All animals showed bouts of hypothermia that became progressively deeper and longer as fasting progressed. EEG and EMG were markedly affected by hypothermia, although the typical electrophysiological signatures of non-rapid eye movement (NREM) sleep, rapid eye movement (REM) sleep, and wakefulness enabled us to perform vigilance-state classification in all cases. Consistent with previous studies, hypothermic bouts were initiated from a state indistinguishable from NREM sleep, with EEG power decreasing gradually in parallel with decreasing surface body temperature. During deep hypothermia, REM sleep was largely abolished, and we observed shivering-associated intense bursts of muscle activity. Our study highlights important similarities between EEG signatures of fasting-induced torpor in mice, daily torpor in Djungarian hamsters and hibernation in seasonally hibernating species. Future studies are necessary to clarify the effects on fasting-induced torpor on subsequent sleep.
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影响因子：
16.6
作者：
Fisher, Simon P.;Cui, Nanyi;McKillop, Laura E.;Gemignani, Jessica;Bannerman, David M.;Oliver, Peter L.;Peirson, Stuart N.;Vyazovskiy, Vladyslav V.
通讯作者：
Vyazovskiy, Vladyslav V.
影响因子：
2.5
作者：
DAAN, S;BARNES, BM;STRIJKSTRA, AM
通讯作者：
STRIJKSTRA, AM
影响因子：
4.4
作者：
Deboer, T
通讯作者：
Deboer, T
影响因子：
2.5
作者：
DEBOER, T;TOBLER, I
通讯作者：
TOBLER, I
影响因子：
--
作者：
FLORANT, GL;TURNER, BM;HELLER, HC
通讯作者：
HELLER, HC