Murine neutrophils present Class II restricted antigen.
Murine neutrophils present Class II restricted antigen.
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DOI：
10.1016/j.imlet.2008.02.008
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发表时间：
2008-06-15
影响因子：
4.4
通讯作者：
McInnes, Iain B.
中科院分区：
文献类型：
--
作者：
Culshaw, Shauna;Millington, Owain R.;Brewer, James M.;McInnes, Iain B.
中性粒细胞最初被描述为寿命短的终末分化的吞噬细胞，其仅对先天性免疫应答起作用。最近的证据表明，中性粒细胞的细胞因子的生产和表达的许多细胞表面蛋白表明，中性粒细胞可能会影响适应性反应，并可能满足抗原呈递细胞的标准。在某些炎症条件下，人中性粒细胞表达主要组织相容性复合体（MHC）II类和共刺激分子CD 80和CD 86。我们采用了鼠T细胞杂交瘤与特异性卵清蛋白肽323-339（OVA 323 -339）的转基因T细胞受体，和一个绿色荧光报告的T细胞受体连接，直接研究嗜中性粒细胞-T细胞的相互作用。这些细胞提供了一个理想的模型系统，允许精确识别抗原特异性和清晰的T细胞活化读数。此外，虽然鼠嗜中性粒细胞先前已显示刺激MHC I类依赖性CD 8 + T细胞活化，但尚未研究通过表达MHC II类嗜中性粒细胞的CD 4 + T细胞刺激。我们通过分离鼠嗜中性粒细胞，加载OVA 323 -339并与对OVA 323 -339/MHC II类复合物特异性的T细胞共培养来解决这一问题，这导致T细胞活化，如通过绿色荧光蛋白报告基因的表达所确定的。抗原脉冲的中性粒细胞也能够以接触依赖性方式引发幼稚的OVA特异性CD 4 + T细胞，如增殖和细胞因子产生所示。淋巴细胞的活化不是由于污染的巨噬细胞。这些研究表明，鼠嗜中性粒细胞呈递MHC II类限制性肽并诱导T细胞增殖，证实了人嗜中性粒细胞中的发现，并证明了鼠嗜中性粒细胞的新型促炎作用。
Neutrophils were originally described as short lived, terminally differentiated phagocytes that contribute only to the innate immune response. Recent evidence of neutrophil cytokine production and expression of numerous cell surface proteins has suggested that neutrophils are likely to influence adaptive responses and may satisfy the criteria of antigen presenting cells. Under certain inflammatory conditions human neutrophils express major histocompatibilty complex (MHC) Class II and the costimulatory molecules CD80 and CD86. We have employed a murine T cell hybridoma with a transgenic T cell receptor specific for ovalbumin peptide 323–339 (OVA323–339), and a green fluorescent reporter of T cell receptor ligation, to directly investigate neutrophil-T cell interactions. These cells provide an ideal model system, allowing precise identification of antigen specificity and a clear readout of T cell activation. Additionally, whilst murine neutrophils have previously been shown to stimulate MHC Class I-dependent CD8+ T cell activation, CD4+ T cells stimulation via MHC Class II-expressing neutrophils has not been investigated. We addressed this by isolating murine neutrophils, loading with OVA323–339 and co-culturing with T cells specific for the OVA323–339/MHC Class II complex, and this resulted in T cell activation, as determined by expression of the green-fluorescent protein reporter. Antigen-pulsed neutrophils were also able to prime naïve OVA-specific CD4+ T cells in a contact-dependent manner, as shown by proliferation and cytokine production. Activation of lymphocytes was not due to contaminating macrophages. These studies demonstrate that murine neutrophils present MHC Class II-restricted peptides and induce T cell proliferation, confirming findings in human neutrophils, and demonstrate a novel pro inflammatory effect of murine neutrophils.
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DOI：
10.1084/jem.187.7.1019
发表时间：
1998-04-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Oehler L;Majdic O;Pickl WF;Stöckl J;Riedl E;Drach J;Rappersberger K;Geissler K;Knapp W
通讯作者：
Knapp W
影响因子：
6.4
作者：
Sandilands, Gavin P.;McCrae, Jame;Baxter, Derek
通讯作者：
Baxter, Derek
影响因子：
--
作者：
Cross, A;Bucknall, RC;Moots, RJ
通讯作者：
Moots, RJ
影响因子：
6.4
作者：
Sandilands, GP;Ahmed, Z;Young, B
通讯作者：
Young, B
DOI：
10.1016/0167-5699(95)80066-2
发表时间：
1995-01-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
CASSATELLA, MA
通讯作者：
CASSATELLA, MA