Variability of the mc1r gene in melanic and non-melanic Podarcis lilfordi and Podarcis pityusensis from the Balearic archipelago.
Variability of the mc1r gene in melanic and non-melanic Podarcis lilfordi and Podarcis pityusensis from the Balearic archipelago.
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来自巴利阿里群岛的黑人和非甲状腺podarcis lilfordi和podarcis pityusensis中MC1R基因的变异性。
DOI：
10.1371/journal.pone.0053088
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ramon MM
中科院分区：
文献类型：
--
作者：
Buades JM;Rodríguez V;Terrasa B;Pérez-Mellado V;Brown RP;Castro JA;Picornell A;Ramon MM
研究了来自巴利阿里群岛的两种墙蜥蜴（Podarcislilfordi和P. pityusensis）的mc 1 r基因座多态性与颜色变化之间的关联。Podarcis lilfordi包括几个深线粒体谱系，其中最古老的起源于上新世，而在P. pityusensis中发现了更浅的线粒体谱系。在这里，我们研究了特定的替代是否与这些物种的胰岛种群中发现的黑色素着色。从来自不同种群的黑质和非黑质Podarcis的73个个体中获得了覆盖大部分mc 1 r基因的同源核序列（还在6个选定的个体中对整个基因进行了测序）。MtDNA基因树也被构建并用作评估mc 1 r多样性的框架。Mc 1 r基因在松叶松中的多态性高于松叶松。然而，我们没有观察到这两个物种中所有黑色素个体所共有的替换。只有一个显着的关联检测到mc 1 r部分序列，但这是一个同义的A/G突变与A等位基因更丰富的黑色素人群。此外，主要显性表型（绿色和棕色，蓝色和黄色的斑点和腹部的颜色）之间没有关联，在mc 1 r基因的同义或非同义取代。在mc 1 r上没有选择的统计学证据。这项研究表明，在巴利阿里Podarcis的mc 1 r多态性和颜色变化之间没有关系。
The association between polymorphism at the mc1r locus and colour variation was studied in two wall lizard species (Podarcis lilfordi and P. pityusensis) from the Balearic archipelago. Podarcis lilfordi comprises several deep mitochondrial lineages, the oldest of which originated in the Pliocene, while much shallower mitochondrial lineages are found in P. pityusensis. Here, we examined whether specific substitutions were associated with the melanic colouration found in islet populations of these species. Homologous nuclear sequences covering most of the mc1r gene were obtained from 73 individuals from melanic and non-melanic Podarcis from different populations (the entire gene was also sequenced in six selected individuals). MtDNA gene trees were also constructed and used as a framework to assess mc1r diversity. Mc1r showed greater polymorphism in P. lilfordi than in P. pityusensis. However, we observed no substitutions that were common to all melanic individuals across the two species. Only one significant association was detected in the mc1r partial sequence, but this was a synonymous A/G mutation with A alleles being more abundant in melanic populations. In addition, there were no associations between the main dominant phenotypes (green and brown, blue and yellow spots and ventral colour) and synonymous or non-synonymous substitutions in the mc1r gene. There was no statistical evidence of selection on mc1r. This study suggests no relationship between mc1r polymorphism and colour variation in Balearic Podarcis.
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DOI：
10.1126/science.1165448
发表时间：
2009-03-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Anderson TM;vonHoldt BM;Candille SI;Musiani M;Greco C;Stahler DR;Smith DW;Padhukasahasram B;Randi E;Leonard JA;Bustamante CD;Ostrander EA;Tang H;Wayne RK;Barsh GS
通讯作者：
Barsh GS
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
3.7
作者：
Micheletti, Steven;Parra, Eliseo;Routman, Eric J.
通讯作者：
Routman, Eric J.
影响因子：
0.7
作者：
Herczeg, Gabor;Matsuba, Chikako;Merila, Juha
通讯作者：
Merila, Juha
影响因子：
2.4
作者：
Kerje, S;Lind, J;Andersson, L
通讯作者：
Andersson, L