Evaluation of immune protection induced by DNA vaccines from Haemaphysalis longicornis paramyosin in rabbits.
Evaluation of immune protection induced by DNA vaccines from Haemaphysalis longicornis paramyosin in rabbits.
复制标题
长角血蜱副肌球蛋白DNA疫苗对家兔免疫保护作用的评价
DOI：
10.1186/s13071-017-2262-x
复制
发表时间：
2017-07-06
影响因子：
3.2
通讯作者：
Hu YH
中科院分区：
文献类型：
--
作者：
Zhang TT;Zhang JC;Cui XJ;Zheng JJ;Li R;Wang F;Liu JZ;Hu YH
研究背景：长角血蜱是一种吸血的体外寄生虫，可通过传播病原体引起人畜疾病。副肌球蛋白（Paramyosin，Pmy）是一种免疫调节蛋白，在机体抗寄生虫的免疫反应中起重要作用。在这项研究中，我们评估了重组质粒编码H。结果：pcDNA3.1（+）-Pmy免疫家兔后产生了较高水平的IgG，提示免疫诱导了体液免疫应答。第4天有部分雌成虫死亡，pcDNA3.1（+）-Pmy组死亡率（27.31%）显著高于对照组（P < 0.0001）。结果表明，pcDNA3.1（+）-Pmy组雌蜱的平均饱腹体重和产卵量分别为109.61 ± 4.24 mg和48.39 ± 4.06 mg，分别比对照组减少36%和39%（P < 0.0001）。结论：Pmy质粒DNA疫苗能诱导有效的体液免疫应答，对H.长角线虫感染
Background:Haemaphysalis longicornis is a blood-sucking ectoparasite that can cause diseases by transmitting some pathogens to humans and animals. Paramyosin (Pmy) is an immunomodulatory protein, which plays an important role in immune reactions against parasites. In this study, we evaluated the immune protection elicited by recombinant plasmids encoding H. longicornis Pmy in rabbits.Results:Rabbits vaccinated with pcDNA3.1(+)-Pmy developed high level of IgG compared to control group, suggesting that humoral immune response was induced by vaccination. On the fourth day after fed on the rabbit, some female adults died and the mortality rate from pcDNA3.1(+)-Pmy group (27.31%) was significantly higher than that of the control group (P < 0.0001). Other female ticks were attached to the rabbits until detachment, and the average engorgement weight, oviposition of female adult from pcDNA3.1(+)-Pmy group were 109.61 ± 4.24 mg and 48.39 ± 4.06 mg, respectively, which correspondingly resulted in 36 and 39% reduction compared with that of the control group (P < 0.0001). In brief, vaccination with Pmy plasmid DNA provided an overall efficacy of 50% in immune protection of rabbits.Conclusions:This study suggested that Pmy DNA vaccine can induce effective humoral immune response and partially protected rabbit against H. longicornis infection.
登录
查看更多内容
口服疫苗接种减毒鼠伤寒沙门氏菌 TsPmy DNA 疫苗可在 BALB/c 小鼠中引发针对旋毛虫的保护性免疫
影响因子：
3.8
作者：
Wang L;Wang X;Bi K;Sun X;Yang J;Gu Y;Huang J;Zhan B;Zhu X
通讯作者：
Zhu X
影响因子：
2.2
作者：
Liu, JZ;Liu, ZN;Gao, ZH
通讯作者：
Gao, ZH
影响因子：
5.5
作者：
Li, BW;Zhang, SR;Weil, GJ
通讯作者：
Weil, GJ
DOI：
10.3347/kjp.2013.51.3.327
发表时间：
2013-06
期刊：
The Korean journal of parasitology
影响因子：
--
作者：
Hu Y;Zhang J;Yang S;Wang H;Zeng H;Zhang T;Liu J
通讯作者：
Liu J
DOI：
10.1126/science.aai9137
发表时间：
2016-10-14
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dowd KA;Ko SY;Morabito KM;Yang ES;Pelc RS;DeMaso CR;Castilho LR;Abbink P;Boyd M;Nityanandam R;Gordon DN;Gallagher JR;Chen X;Todd JP;Tsybovsky Y;Harris A;Huang YS;Higgs S;Vanlandingham DL;Andersen H;Lewis MG;De La Barrera R;Eckels KH;Jarman RG;Nason MC;Barouch DH;Roederer M;Kong WP;Mascola JR;Pierson TC;Graham BS
通讯作者：
Graham BS