Decoding the ubiquitin-mediated pathway of arthropod disease vectors.
Decoding the ubiquitin-mediated pathway of arthropod disease vectors.
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解码泛素介导的节肢动物疾病载体的途径。
DOI：
10.1371/journal.pone.0078077
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Pedra JH
中科院分区：
文献类型：
--
作者：
Choy A;Severo MS;Sun R;Girke T;Gillespie JJ;Pedra JH
泛素对蛋白质的调节在模式生物中得到了广泛的描述。然而，泛素机制在疾病媒介中的特征仍然大部分是未知的。这一认识上的根本差距令人担忧，因为需要新的疗法来控制媒介传播的疾病，而将泛素机制作为疾病干预的一种手段已经在临床上采用。在这项研究中，我们利用生物信息学的方法，在冈比亚按蚊、埃及伊蚊、致倦库蚊、肩部硬蜱、人血库蚊和罗德尼库蚊的基因组中揭示了泛素介导的途径。我们观察到：(1)与果蝇、小鼠和智人相比，疾病载体编码泛素相关基因的比例较低，但与酿酒酵母相比，泛素相关基因的编码比例较低；(2)总体而言，与E2结合酶或E1激活酶相比，存在更多属于E3泛素连接酶的蛋白质；(3)三种蚊子基因组中的泛素机制非常相似；(4)与其他节肢动物载体相比，查加斯病载体(R.prolixus)中泛素基因的比例增加了一倍以上；(5)鹿体虱和体虱(人虱)基因组携带少量的E1激活酶和Hect类型的E3泛素连接酶；(6)普氏扁虱具有少量的环型E3泛素连接酶；(7)致倦库蚊具有较高数量的预测的F-box E3泛素连接酶、JAB和UCH脱泛素连接酶。综上所述，这些发现为研究病原体和节肢动物媒介之间的相互作用提供了新的机会。
Protein regulation by ubiquitin has been extensively described in model organisms. However, characterization of the ubiquitin machinery in disease vectors remains mostly unknown. This fundamental gap in knowledge presents a concern because new therapeutics are needed to control vector-borne diseases, and targeting the ubiquitin machinery as a means for disease intervention has been already adopted in the clinic. In this study, we employed a bioinformatics approach to uncover the ubiquitin-mediated pathway in the genomes of Anopheles gambiae, Aedes aegypti, Culex quinquefasciatus, Ixodes scapularis, Pediculus humanus and Rhodnius prolixus. We observed that (1) disease vectors encode a lower percentage of ubiquitin-related genes when compared to Drosophila melanogaster, Mus musculus and Homo sapiens but not Saccharomyces cerevisiae; (2) overall, there are more proteins categorized as E3 ubiquitin ligases when compared to E2-conjugating or E1-activating enzymes; (3) the ubiquitin machinery within the three mosquito genomes is highly similar; (4) ubiquitin genes are more than doubled in the Chagas disease vector (R. prolixus) when compared to other arthropod vectors; (5) the deer tick I. scapularis and the body louse (P. humanus) genomes carry low numbers of E1-activating enzymes and HECT-type E3 ubiquitin ligases; (6) R. prolixus have low numbers of RING-type E3 ubiquitin ligases; and (7) C. quinquefasciatus present elevated numbers of predicted F-box E3 ubiquitin ligases, JAB and UCH deubiquitinases. Taken together, these findings provide novel opportunities to study the interaction between a pathogen and an arthropod vector.
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DOI：
10.1126/science.1193162
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bartholomay LC;Waterhouse RM;Mayhew GF;Campbell CL;Michel K;Zou Z;Ramirez JL;Das S;Alvarez K;Arensburger P;Bryant B;Chapman SB;Dong Y;Erickson SM;Karunaratne SH;Kokoza V;Kodira CD;Pignatelli P;Shin SW;Vanlandingham DL;Atkinson PW;Birren B;Christophides GK;Clem RJ;Hemingway J;Higgs S;Megy K;Ranson H;Zdobnov EM;Raikhel AS;Christensen BM;Dimopoulos G;Muskavitch MA
通讯作者：
Muskavitch MA
Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
9.5
作者：
Cooper, Peter S.;Lipshultz, Dawn;Sayers, Eric W.
通讯作者：
Sayers, Eric W.
影响因子：
4.4
作者：
David, Jean-Philippe;Coissac, Eric;Reynaud, Stephane
通讯作者：
Reynaud, Stephane
影响因子：
11.4
作者：
Cooper, Eric M.;Cutcliffe, Colleen;Cohen, Robert E.
通讯作者：
Cohen, Robert E.