Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress.
Identification of a DNA-binding site for the transcription factor Haa1, required for Saccharomyces cerevisiae response to acetic acid stress.
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DOI：
10.1093/nar/gkr228
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发表时间：
2011-09-01
影响因子：
14.9
通讯作者：
Sá-Correia I
中科院分区：
文献类型：
--
作者：
Mira NP;Henriques SF;Keller G;Teixeira MC;Matos RG;Arraiano CM;Winge DR;Sá-Correia I
转录因子Haa 1是响应乙酸应激重新编程酵母基因组表达的主要参与者。映射的启动子区域的一个Haa 1激活的基因，TPO 3，允许识别的乙酸响应元件（ACRE），Haa 1结合在体内。对Haa 1调节子基因启动子区的计算机分析鉴定出Haa 1反应元件（HRE）5′-GNN（G/C）（A/C）（A/G）G（A/G/C）G-3′。使用表面等离子体共振实验和电泳迁移率变动分析，它表明，Haa 1相互作用的高亲和力（KD为2 nM）与HRE基序存在于TPO 3启动子的ACRE区域。在Haa 1和在位置6和8具有腺嘌呤核苷酸的HRE基序之间没有发现显著的相互作用（KD分别为396和6780 nM），这表明Haa 1 p在体内不识别这些基序。还观察到Haa 1对在位置7和9的鸟嘌呤核苷酸中具有突变的HRE基序的较低亲和力（KD分别为21和119 nM）。Haa 1的最小功能结合位点为5′-（G/C）（A/C）GG（G/C）G-3′。Haa 1依赖的转录调控网络活性酵母响应乙酸胁迫的建议。
The transcription factor Haa1 is the main player in reprogramming yeast genomic expression in response to acetic acid stress. Mapping of the promoter region of one of the Haa1-activated genes, TPO3, allowed the identification of an acetic acid responsive element (ACRE) to which Haa1 binds in vivo. The in silico analysis of the promoter regions of the genes of the Haa1-regulon led to the identification of an Haa1-responsive element (HRE) 5′-GNN(G/C)(A/C)(A/G)G(A/G/C)G-3′. Using surface plasmon resonance experiments and electrophoretic mobility shift assays it is demonstrated that Haa1 interacts with high affinity (KD of 2 nM) with the HRE motif present in the ACRE region of TPO3 promoter. No significant interaction was found between Haa1 and HRE motifs having adenine nucleotides at positions 6 and 8 (KD of 396 and 6780 nM, respectively) suggesting that Haa1p does not recognize these motifs in vivo. A lower affinity of Haa1 toward HRE motifs having mutations in the guanine nucleotides at position 7 and 9 (KD of 21 and 119 nM, respectively) was also observed. Altogether, the results obtained indicate that the minimal functional binding site of Haa1 is 5′-(G/C)(A/C)GG(G/C)G-3′. The Haa1-dependent transcriptional regulatory network active in yeast response to acetic acid stress is proposed.
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影响因子：
6.4
作者：
Mira NP;Palma M;Guerreiro JF;Sá-Correia I
通讯作者：
Sá-Correia I
影响因子：
4.8
作者：
BACHHAWAT, N;OUYANG, QA;HENRY, SA
通讯作者：
HENRY, SA
DOI：
10.1073/pnas.86.1.65
发表时间：
1989-01-01
影响因子：
11.1
作者：
HUIBREGTSE, JM;ENGELKE, DR;THIELE, DJ
通讯作者：
THIELE, DJ
影响因子：
5.3
作者：
Lucau-Danila, A;Lelandais, G;Jacq, C
通讯作者：
Jacq, C
DOI：
10.1073/pnas.0509185103
发表时间：
2006-06-27
影响因子：
11.1
作者：
Ho, Su-Wen;Jona, Ghil;Snyder, Michael
通讯作者：
Snyder, Michael