Identification and utilization of a sow thistle powdery mildew as a poorly adapted pathogen to dissect post-invasion non-host resistance mechanisms in Arabidopsis.
Identification and utilization of a sow thistle powdery mildew as a poorly adapted pathogen to dissect post-invasion non-host resistance mechanisms in Arabidopsis.
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猪蓟白粉病作为一种适应性较差的病原体的鉴定和利用，以剖析拟南芥入侵后非宿主抗性机制
DOI：
10.1093/jxb/erq406
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发表时间：
2011-03
影响因子：
6.9
通讯作者：
Xiao S
中科院分区：
文献类型：
--
作者：
Wen Y;Wang W;Feng J;Luo MC;Tsuda K;Katagiri F;Bauchan G;Xiao S
为了更好地剖析对形成吸器的白粉病病原体的非宿主抗性，鉴定了一种被命名为Golovinomyces cichoracearum UMSG1的猪蓟白粉病分离株，它在很大程度上克服了渗透抗性，但总是被拟南芥入侵后非宿主抗性所阻止。入侵后的非宿主抵抗主要表现为吸器复合体（EHC）和过敏反应（HR）的胼胝体包裹的形成，这似乎受到水杨酸（SA）依赖性和SA独立性防御途径的控制，这得到pad4/sid2双突变体对病原体的敏感性的支持。虽然广谱抗性蛋白 RPW8.2 增强了对菊苣 G. cichoracearum UCSC1 的渗透后抗性，但适应性良好的白粉病病原体 RPW8.2 对于 G. cichoracearum UMSG1 的渗透后抗性来说是可有可无的，并且其对吸器外膜的特异性靶向被 EHC 物理阻断，导致 HR 细胞死亡。综上所述，目前的工作提出了拟南芥与白粉病相互作用的进化情景：EHC 的形成是植物中针对吸器入侵而进化的一种保守的亚细胞防御；适应性强的白粉病已进化出抑制 EHC 形成以促进寄生生长和繁殖的能力； RPW8.2 已进化为增强 EHC 形成，从而在入侵后阶段赋予吸器靶向的广谱抗性。
To better dissect non-host resistance against haustorium-forming powdery mildew pathogens, a sow thistle powdery mildew isolate designated Golovinomyces cichoracearum UMSG1 that has largely overcome penetration resistance but is invariably stopped by post-invasion non-host resistance of Arabidopsis thaliana was identified. The post-invasion non-host resistance is mainly manifested as the formation of a callosic encasement of the haustorial complex (EHC) and hypersensitive response (HR), which appears to be controlled by both salicylic acid (SA)-dependent and SA-independent defence pathways, as supported by the susceptibility of the pad4/sid2 double mutant to the pathogen. While the broad-spectrum resistance protein RPW8.2 enhances post-penetration resistance against G. cichoracearum UCSC1, a well-adapted powdery mildew pathogen, RPW8.2, is dispensable for post-penetration resistance against G. cichoracearum UMSG1, and its specific targeting to the extrahaustorial membrane is physically blocked by the EHC, resulting in HR cell death. Taken together, the present work suggests an evolutionary scenario for the Arabidopsis–powdery mildew interaction: EHC formation is a conserved subcellular defence evolved in plants against haustorial invasion; well-adapted powdery mildew has evolved the ability to suppress EHC formation for parasitic growth and reproduction; RPW8.2 has evolved to enhance EHC formation, thereby conferring haustorium-targeted, broad-spectrum resistance at the post-invasion stage.
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影响因子：
3.5
作者：
Mellersh, DG;Heath, MC
通讯作者：
Heath, MC
影响因子：
64.8
作者：
Collins, NC;Thordal-Christensen, H;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P
影响因子：
7.2
作者：
Adam, L;Somerville, SC
通讯作者：
Somerville, SC
影响因子：
3.2
作者：
Heath, MC;Xu, HX;Eilam, T
通讯作者：
Eilam, T
影响因子：
56.9
作者：
Alonso, JM;Hirayama, T;Ecker, JR
通讯作者：
Ecker, JR