Cardiac Extracellular Matrix Modification as a Therapeutic Approach.
Cardiac Extracellular Matrix Modification as a Therapeutic Approach.
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DOI：
10.1007/978-3-319-97421-7_7
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发表时间：
2018
影响因子：
--
通讯作者：
Ogle BM
中科院分区：
文献类型：
--
作者：
Hall ML;Ogle BM
心脏细胞外基质 (cECM) 由心脏细胞类型分泌的蛋白质和多糖组成，为心血管组织提供结构和生化支持。 cECM 蛋白和相关细胞表面受体家族整合素的作用已通过生成敲除实验动物模型在体内进行了探索。然而，组织的复杂性使得很难分离单个 cECM 蛋白对特定细胞过程或疾病状态的影响。进一步剖析 cECM 作用的愿望导致了各种体外模型系统的开发，这些系统现在不仅用于基础研究，还用于测试药物功效和毒性以及生成治疗支架。这些系统始于源自组织的 cECM 二维涂层，现已发展到包括重组 ECM 蛋白、ECM 片段和 ECM 模拟物。最近出现了 3D 模型系统，多种开发技术使之成为可能，其中最引人注目的是 3D 生物打印。本章将尝试追踪我们对 cECM 与细胞行为之间关系的理解从体内模型到体外控制系统的演变。我们在本章的最后总结了诸如此类的基础研究如何指导使用 cECM 作为直接疗法。
The cardiac extracellular matrix (cECM) is comprised of proteins and polysaccharides secreted by cardiac cell types, which provide structural and biochemical support to cardiovascular tissue. The roles of cECM proteins and the associated family of cell surface receptor, integrins, have been explored in vivo via the generation of knockout experimental animal models. However, the complexity of tissues makes it difficult to isolate the effects of individual cECM proteins on a particular cell process or disease state. The desire to further dissect the role of cECM has led to the development of a variety of in vitro model systems, which are now being used not only for basic studies but also for testing drug efficacy and toxicity and for generating therapeutic scaffolds. These systems began with 2D coatings of cECM derived from tissue and have developed to include recombinant ECM proteins, ECM fragments, and ECM mimics. Most recently 3D model systems have emerged, made possible by several developing technologies including, and most notably, 3D bioprinting. This chapter will attempt to track the evolution of our understanding of the relationship between cECM and cell behavior from in vivo model to in vitro control systems. We end the chapter with a summary of how basic studies such as these have informed the use of cECM as a direct therapy.
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影响因子：
13.6
作者：
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通讯作者：
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影响因子：
9.7
作者：
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通讯作者：
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2.4
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影响因子：
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通讯作者：
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