Chromatin state changes during neural development revealed by in vivo cell-type specific profiling.
Chromatin state changes during neural development revealed by in vivo cell-type specific profiling.
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DOI：
10.1038/s41467-017-02385-4
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发表时间：
2017-12-22
影响因子：
16.6
通讯作者：
Brand AH
中科院分区：
文献类型：
--
作者：
Marshall OJ;Brand AH
发育生物学的一个关键问题是发育过程中如何控制细胞分化。虽然三胸群 (TrxG) 和多梳群 (PcG) 染色质状态之间的转变对于 ES 细胞向多能干细胞的分化至关重要，但人们对染色质状态在大脑发育过程中的作用知之甚少。在这里，我们展示了果蝇神经发育过程中发生的大规模染色质重塑。我们证明，神经元分化过程中激活的大多数基因在神经干细胞 (NSC) 中是沉默的，并占据黑色染色质和 TrxG 抑制状态。在神经元中，几乎所有关键的 NSC 基因都通过 HP1 介导的抑制而关闭。 PcG 介导的抑制在调节这些基因方面并不发挥重要作用，而是调节控制大脑空间和时间模式的谱系特异性转录因子。综合起来，我们的数据表明，除了典型的 PcG/TrxG 转变之外的染色质形式在神经发育过程中发挥着关键作用。虽然活跃和抑制染色质状态之间的转变对于分化至关重要，但人们对它们在果蝇大脑发育过程中的作用知之甚少。在这里，作者研究了果蝇神经发育过程中发生的大规模染色质重塑。
A key question in developmental biology is how cellular differentiation is controlled during development. While transitions between trithorax-group (TrxG) and polycomb-group (PcG) chromatin states are vital for the differentiation of ES cells to multipotent stem cells, little is known regarding the role of chromatin states during development of the brain. Here we show that large-scale chromatin remodelling occurs during Drosophila neural development. We demonstrate that the majority of genes activated during neuronal differentiation are silent in neural stem cells (NSCs) and occupy black chromatin and a TrxG-repressive state. In neurons, almost all key NSC genes are switched off via HP1-mediated repression. PcG-mediated repression does not play a significant role in regulating these genes, but instead regulates lineage-specific transcription factors that control spatial and temporal patterning in the brain. Combined, our data suggest that forms of chromatin other than canonical PcG/TrxG transitions take over key roles during neural development. While transitions between active and repressive chromatin states are essential for differentiation, little is known regarding their role during development of the brain in Drosophila. Here, the authors investigate the large scale chromatin remodelling taking place during fly neural development.
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影响因子：
3.6
作者：
Gold KS;Brand AH
通讯作者：
Brand AH
影响因子：
30.8
作者：
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64.8
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作者：
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