Evidence that mating by the Saccharomyces cerevisiae gpa1Val50 mutant occurs through the default mating pathway and a suggestion of a role for ubiquitin-mediated proteolysis.
Evidence that mating by the Saccharomyces cerevisiae gpa1Val50 mutant occurs through the default mating pathway and a suggestion of a role for ubiquitin-mediated proteolysis.
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有证据表明酿酒酵母 gpa1Val50 突变体通过默认交配途径发生交配，并暗示泛素介导的蛋白水解作用。
DOI：
10.1091/mbc.8.9.1649
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发表时间：
1997
影响因子：
3.3
通讯作者：
Kurjan,J
中科院分区：
文献类型：
--
作者：
Xu,BE;Kurjan,J
酵母G α亚基Gpa 1 p在信息素反应途径中起着负作用。gpa 1Val 50突变体先前被证明具有生长缺陷，与该突变预测的GTdR缺陷一致，并且大大减少了交配。对于这种配合缺陷，人们提出了各种各样的解释。分析gpa 1Val 50交配缺陷的一种方法涉及上位性分析。低交配的gpa 1Val 50突变体是独立的信息素受体，因此，它的结果从细胞内激活的途径，符合GTdR缺陷。这一结果表明，gpa 1Val 50交配发生通过默认而不是参与信息素反应的趋化途径。因此，我们测试了spa 2突变对gpa 1Val 50交配的影响，因为Spa 2 p与默认途径有关。spa 2突变大大减少了交配的gpa 1Val 50突变体，表明gpa 1Val 50交配主要通过默认途径发生。在研究gpa 1Val 50表型的第二种方法中，分离了gpa 1Val 50交配缺陷的抑制因子。两个抑制基因对应于SON 1/UFD 5和SEN 3，它们与泛素介导的蛋白水解有关。在这些结果的基础上，我们认为，一个积极的组成部分的默认交配途径是受泛素介导的降解。
The yeast G alpha subunit, Gpa1p, plays a negative role in the pheromone response pathway. The gpa1Val50 mutant was previously shown to have a growth defect, consistent with the GTPase defect predicted for this mutation, and greatly reduced mating. Various explanations for the mating defect have been proposed. One approach to analyze the gpa1Val50 mating defect involved epistasis analysis. The low mating of the gpa1Val50 mutant was independent of the pheromone receptor; therefore, it results from intracellular activation of the pathway, consistent with a GTPase defect. This result suggests that gpa1Val50 mating occurs through the default rather than the chemotropic pathway involved in pheromone response. We therefore tested the effect of a spa2 mutation on gpa1Val50 mating, because Spa2p has been implicated in the default pathway. The spa2 mutation greatly reduced the mating of the gpa1Val50 mutant, suggesting that gpa1Val50 mating occurs predominantly through the default pathway. In a second approach to investigate the gpa1Val50 phenotypes, suppressors of the gpa1Val50 mating defect were isolated. Two suppressor genes corresponded to SON1/UFD5 and SEN3, which are implicated in ubiquitin-mediated proteolysis. On the basis of these results, we suggest that a positive component of the default mating pathway is subject to ubiquitin-mediated degradation.
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DOI：
10.1073/pnas.88.21.9392
发表时间：
1991-11-01
影响因子：
11.1
作者：
ELION, EA;BRILL, JA;FINK, GR
通讯作者：
FINK, GR
影响酿酒酵母 tRNA 剪接核酸内切酶活性的突变。
影响因子：
3.3
作者：
Mark Winey;M. Culbertson
通讯作者：
M. Culbertson
STE4基因的过度表达导致单倍体酿酒酵母的交配反应
影响因子：
5.3
作者：
M. Whiteway;L. Hougan;D. Thomas
通讯作者：
D. Thomas
影响因子：
--
作者：
Ira Herskowitz;Robert Jensen
通讯作者：
Ira Herskowitz;Robert Jensen
DOI：
10.1083/jcb.131.4.863
发表时间：
1995-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Valtz N;Peter M;Herskowitz I
通讯作者：
Herskowitz I