The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti.
The sequence of a male-specific genome region containing the sex determination switch in Aedes aegypti.
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DOI：
10.1186/s13071-018-3090-3
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发表时间：
2018-10-20
影响因子：
3.2
通讯作者：
Darby AC
中科院分区：
文献类型：
--
作者：
Turner J;Krishna R;Van't Hof AE;Sutton ER;Matzen K;Darby AC
埃及伊蚊是几种重要虫媒病毒的主要媒介。控制病媒以限制疾病传播的方法包括释放不育或转基因的不叮咬雄性蚊子的遗传策略，这引起了人们对操纵蚊子性别比例的兴趣。伊蚊的性别决定埃及伊蚊是由一个叫做M位点的非重组Y染色体样区域控制的，然而这个位点的特征被基因组的重复特性所阻碍。2015年，一个名为Nix的M位点基因被发现，它显示了性别决定开关的特性。利用全基因组细菌人工染色体（BAC）文库，对含有雄性决定基因Nix的约200 kb区域进行了扩增和测序。在这项研究中，我们发现Nix由两个外显子组成，其中99kb的内含子主要由重复DNA组成，特别是转座元件。Nix是一个异常大且高度重复的基因，在其他生物体中表现出与Y染色体基因相同的特征。我们推测，M位点缺乏重组使得重复序列以一种性别限制染色体的方式扩展，这与提出的昆虫性染色体进化模型相一致。本文的在线版本（10.1186/s13071-018-3090-3）包含补充材料，授权用户可使用。
Aedes aegypti is the principal vector of several important arboviruses. Among the methods of vector control to limit transmission of disease are genetic strategies that involve the release of sterile or genetically modified non-biting males, which has generated interest in manipulating mosquito sex ratios. Sex determination in Ae. aegypti is controlled by a non-recombining Y chromosome-like region called the M locus, yet characterisation of this locus has been thwarted by the repetitive nature of the genome. In 2015, an M locus gene named Nix was identified that displays the qualities of a sex determination switch. With the use of a whole-genome bacterial artificial chromosome (BAC) library, we amplified and sequenced a ~200 kb region containing the male-determining gene Nix. In this study, we show that Nix is comprised of two exons separated by a 99 kb intron primarily composed of repetitive DNA, especially transposable elements. Nix, an unusually large and highly repetitive gene, exhibits features in common with Y chromosome genes in other organisms. We speculate that the lack of recombination at the M locus has allowed the expansion of repeats in a manner characteristic of a sex-limited chromosome, in accordance with proposed models of sex chromosome evolution in insects. The online version of this article (10.1186/s13071-018-3090-3) contains supplementary material, which is available to authorized users.
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影响因子：
2.7
作者：
Gilles, Jeremie R. L.;Schetelig, Marc F.;Bourtzis, Kostas
通讯作者：
Bourtzis, Kostas
影响因子：
3.7
作者：
Lacroix R;McKemey AR;Raduan N;Kwee Wee L;Hong Ming W;Guat Ney T;Rahidah A A S;Salman S;Subramaniam S;Nordin O;Hanum A T N;Angamuthu C;Marlina Mansor S;Lees RS;Naish N;Scaife S;Gray P;Labbé G;Beech C;Nimmo D;Alphey L;Vasan SS;Han Lim L;Wasi A N;Murad S
通讯作者：
Murad S
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z
DOI：
10.1073/pnas.231484998
发表时间：
2001-11-06
影响因子：
11.1
作者：
Carvalho, AB;Dobo, BA;Clark, AG
通讯作者：
Clark, AG
影响因子：
56.9
作者：
CRAIG, GB;HICKEY, WA;VANDEHEY, RC
通讯作者：
VANDEHEY, RC