Open field release of genetically engineered sterile male Aedes aegypti in Malaysia.
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia.
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DOI：
10.1371/journal.pone.0042771
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Murad S
中科院分区：
文献类型：
--
作者：
Lacroix R;McKemey AR;Raduan N;Kwee Wee L;Hong Ming W;Guat Ney T;Rahidah A A S;Salman S;Subramaniam S;Nordin O;Hanum A T N;Angamuthu C;Marlina Mansor S;Lees RS;Naish N;Scaife S;Gray P;Labbé G;Beech C;Nimmo D;Alphey L;Vasan SS;Han Lim L;Wasi A N;Murad S
登革热是最重要的蚊媒病毒性疾病。在缺乏特效药物或疫苗的情况下，控制工作的重点是抑制主要的蚊子媒介-埃及伊蚊，但目前的方法还不足以控制这种疾病。因此，迫切需要新的方法，例如基于遗传学的雄性不育释放方法。然而，这需要实验室饲养的改良蚊子能够在野外存活并充分分散。成年雄性蚊子被释放到马来西亚彭亨无人居住的森林地区。它们的生存和扩散进行了评估，通过使用网络的陷阱。使用了两种菌株，一种工程化的“遗传不育”（OX 513 A）和一种野生型实验室菌株，以提供关于经修饰的蚊子的性能的绝对和相对数据。这两种菌株具有相似的最大传播距离（220米），但OX 513 A菌株的平均传播距离较低（52对100米）。预期寿命相似（2.0 vs 2.2天）。两种菌株的回收率都很高，可能是因为该地点无人居住。经过广泛的控制研究和监管审查，在马来西亚安全成功地进行了工程蚊子的实地释放。工程菌株表现出类似的领域寿命未修改的对应物，虽然在这种设置中的分散相对于未修改的菌株减少。这些数据对于未来遗传控制策略的测试和实施是令人鼓舞的，并将有助于指导这种和其他工程菌株的未来田间使用。
Dengue is the most important mosquito-borne viral disease. In the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, Aedes aegypti, yet current methods have not proven adequate to control the disease. New methods are therefore urgently needed, for example genetics-based sterile-male-release methods. However, this requires that lab-reared, modified mosquitoes be able to survive and disperse adequately in the field. Adult male mosquitoes were released into an uninhabited forested area of Pahang, Malaysia. Their survival and dispersal was assessed by use of a network of traps. Two strains were used, an engineered ‘genetically sterile’ (OX513A) and a wild-type laboratory strain, to give both absolute and relative data about the performance of the modified mosquitoes. The two strains had similar maximum dispersal distances (220 m), but mean distance travelled of the OX513A strain was lower (52 vs. 100 m). Life expectancy was similar (2.0 vs. 2.2 days). Recapture rates were high for both strains, possibly because of the uninhabited nature of the site. After extensive contained studies and regulatory scrutiny, a field release of engineered mosquitoes was safely and successfully conducted in Malaysia. The engineered strain showed similar field longevity to an unmodified counterpart, though in this setting dispersal was reduced relative to the unmodified strain. These data are encouraging for the future testing and implementation of genetic control strategies and will help guide future field use of this and other engineered strains.
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影响因子：
3.7
作者：
Bargielowski I;Alphey L;Koella JC
通讯作者：
Koella JC
影响因子：
3
作者：
Catteruccia F;Crisanti A;Wimmer EA
通讯作者：
Wimmer EA
影响因子：
46.9
作者：
Harris, Angela F.;Nimmo, Derric;Alphey, Luke
通讯作者：
Alphey, Luke
DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Fu, Guoliang;Condon, Kirsty C.;Alphey, Luke
通讯作者：
Alphey, Luke