A Morphometric Investigation of Myotube Formation in Rabbit Embryo Medial Pterygoid Muscle
A Morphometric Investigation of Myotube Formation in Rabbit Embryo Medial Pterygoid Muscle
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兔胚胎翼内肌肌管形成的形态学研究
DOI：
10.1177/00220345960750110401
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发表时间：
1996
影响因子：
7.6
通讯作者：
C. Baudoin
中科院分区：
文献类型：
--
作者：
R. Benoît;C. Baudoin
为了确定成肌细胞、早期肌管、晚期肌管和肌纤维的出现时间，我们在不干扰正常神经支配的情况下，研究了两个品系兔胚胎中翼状内咀嚼肌的两个腱膜之间的区域。本研究的目的是确定这些细胞之间的定量关系，并从统计学上确定它们的动力学。在妊娠第17天、第17天加12小时、第18天、第18天加12小时以及第20、22、28天使用勃艮第和新西兰兔胚胎。光镜下用计数法观察细胞增殖。在两个家兔品系中也观察到类似的发展。成肌细胞数量减少如下：(1)明显减少；（ii）突然停止下降，在第18天出现反弹，持续时间少于24小时；（iii）胚胎第22天和第28天之间的平稳期。早期肌管数量的减少与成肌细胞的反弹一致。晚期肌管数量在早期肌管密度最大时和成肌细胞反弹时增加。第22天肌纤维密度与晚期肌管密度相似。我们认为早期肌管是通过成肌细胞的融合逐渐形成的，而成肌细胞数量的显著增加对应于晚期肌管分化所必需的第二代成肌细胞。关键词：咀嚼肌；模型；统计学；1994年4月18日收到；1996年5月30日接受
To determine the times of the appearance of myoblasts, early myotubes, late myotubes, and myofibers, we studied a region between two aponeuroses of the medial pterygoid masticatory muscle in embryos of two strains of rabbits, without disturbing the normal innervation. The objectives of this study were to define the quantitative relations among these cells and to determine their kinetics statistically. We used Fauve de Bourgogne and New Zealand rabbit embryos on day 17, day 17 plus 12 hours, day 18, day 18 plus 12 hours, and days 20, 22, and 28 of gestation. Cell proliferation was studied with a light microscope, by means of counting methods. Similar development was observed in the two strains of rabbits. The numbers of myoblasts decreased as follows: (i) a marked decrease; (ii) a sudden cessation of the decrease, marked by a rebound at 18 days, and lasting less than 24 hours; and (iii) a plateau between embryonic days 22 and 28. The onset of reduction in the number of early myotubes coincided with the rebound of myoblasts. The number of late myotubes increased at the time of maximal early myotube density and during rebound of the myoblasts. Myofiber densities were similar to late myotube densities on day 22. We suggest that early myotubes are formed very gradually by fusion of myoblasts, and that the significant increase in the numbers of myoblasts corresponds to the second generation of myoblasts necessary for differentiation of late myotubes. Key words: masticatory muscles, models, statistical, rabbits. Received April 18, 1994; Accepted May 30, 1996
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影响因子：
2.7
作者：
Rushbrook,JI;Weiss,C;Yao,TT
通讯作者：
Yao,TT
DOI：
10.1002/jez.1402370206
发表时间：
1986
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
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通讯作者：
Wineski,LE
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DOI：
10.1002/aja.1001810306
发表时间：
1988
期刊：
The American journal of anatomy
影响因子：
--
作者：
Ontell,M;Hughes,D;Bourke,D
通讯作者：
Bourke,D
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影响因子：
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作者：
Stedman,HH;Kelly,AM;Rubinstein,NA
通讯作者：
Rubinstein,NA