Chronic high-fat diet impairs collecting lymphatic vessel function in mice.
Chronic high-fat diet impairs collecting lymphatic vessel function in mice.
复制标题
DOI：
10.1371/journal.pone.0094713
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Detmar M
中科院分区：
文献类型：
--
作者：
Blum KS;Karaman S;Proulx ST;Ochsenbein AM;Luciani P;Leroux JC;Wolfrum C;Detmar M
淋巴管在肠道脂肪摄取中起重要作用，淋巴管功能受损导致淋巴水肿患者和遗传性淋巴功能障碍小鼠脂肪组织堆积增加。然而，肥胖症对淋巴功能的影响研究很少。我们调查了脂肪组织堆积是否以及如何影响淋巴功能。使用淋巴特异性示踪剂，我们在体内进行了近红外(NIR)成像，以评估喂食正常饮食或高脂饮食(HFD)的小鼠收集淋巴管的功能。用组织学和整体切片观察起始淋巴管和集合淋巴管的形态变化。HFD与收集淋巴管功能受损有关，表现为收缩频率减少和对机械刺激的反应减弱。此外，我们还发现收集淋巴管功能与体重之间存在显著的负相关。整体分析显示，后肢的收缩集合淋巴管增大。在K14-VEGF-C小鼠中，HFD导致示踪剂在皮肤淋巴管内的扩散减少。这些发现表明，由于HFD引起的脂肪组织扩张导致淋巴管系统的功能损害，主要是在收集淋巴管方面。
Lymphatic vessels play an essential role in intestinal lipid uptake, and impairment of lymphatic vessel function leads to enhanced adipose tissue accumulation in patients with lymphedema and in genetic mouse models of lymphatic dysfunction. However, the effects of obesity on lymphatic function have been poorly studied. We investigated if and how adipose tissue accumulation influences lymphatic function. Using a lymphatic specific tracer, we performed in vivo near-infrared (NIR) imaging to assess the function of collecting lymphatic vessels in mice fed normal chow or high-fat diet (HFD). Histological and whole mount analyses were performed to investigate the morphological changes in initial and the collecting lymphatic vessels. HFD was associated with impaired collecting lymphatic vessel function, as evidenced by reduced frequency of contractions and diminished response to mechanostimulation. Moreover, we found a significant negative correlation between collecting lymphatic vessel function and body weight. Whole mount analyses showed an enlargement of contractile collecting lymphatic vessels of the hind limb. In K14-VEGF-C mice, HFD resulted in a reduced spreading of the tracer within dermal lymphatic vessels. These findings indicate that adipose tissue expansion due to HFD leads to a functional impairment of the lymphatic vasculature, predominantly in collecting lymphatic vessels.
登录
查看更多内容
影响因子：
2.8
作者：
BILANCINI, S;LUCCHI, M;ELEUTERI, P
通讯作者：
ELEUTERI, P
影响因子：
3.7
作者：
Weitman, Evan S.;Aschen, Seth Z.;Mehrara, Babak J.
通讯作者：
Mehrara, Babak J.
影响因子：
6
作者：
Rutkowski, Joseph M.;Markhus, Carl Erik;Swartz, Melody A.
通讯作者：
Swartz, Melody A.
影响因子：
3.7
作者：
Gogineni A;Caunt M;Crow A;Lee CV;Fuh G;van Bruggen N;Ye W;Weimer RM
通讯作者：
Weimer RM
影响因子：
--
作者：
Zhou, Quan;Wood, Ronald;Schwarz, Edward M.;Wang, Yong-Jun;Xing, Lianping
通讯作者：
Xing, Lianping