Post-transcriptional processing generates a diversity of 5'-modified long and short RNAs.
Post-transcriptional processing generates a diversity of 5'-modified long and short RNAs.
复制标题
DOI：
10.1038/nature07759
复制
发表时间：
2009-02-19
期刊：
影响因子：
64.8
通讯作者：
Gingeras, Thomas R.
中科院分区：
文献类型：
--
作者：
Fejes-Toth, Katalin;Sotirova, Vihra;Sachidanandam, Ravi;Assaf, Gordon;Hannon, Gregory J.;Kapranov, Philipp;Foissac, Sylvain;Willingham, Aarron T.;Duttagupta, Radha;Dumais, Erica;Gingeras, Thomas R.
The transcriptomes of eukaryotic cells are incredibly complex. Individual non-coding RNAs dwarf the number of protein-coding genes, and include classes that are well understood as well as classes for which the nature, extent and functional roles are obscure. Deep sequencing of small RNAs (<200 nucleotides) from human HeLa and HepG2 cells revealed a remarkable breadth of species. These arose both from within annotated genes and from unannotated intergenic regions. Overall, small RNAs tended to align with CAGE (cap-analysis of gene expression) tags, which mark the 5′ ends of capped, long RNA transcripts. Many small RNAs, including the previously described promoter-associated small RNAs, appeared to possess cap structures. Members of an extensive class of both small RNAs and CAGE tags were distributed across internal exons of annotated protein coding and non-coding genes, sometimes crossing exon–exon junctions. Here we show that processing of mature mRNAs through an as yet unknown mechanism may generate complex populations of both long and short RNAs whose apparently capped 5′ ends coincide. Supplying synthetic promoter-associated small RNAs corresponding to the c-MYC transcriptional start site reduced MYC messenger RNA abundance. The studies presented here expand the catalogue of cellular small RNAs and demonstrate a biological impact for at least one class of non-canonical small RNAs.
登录
查看更多内容
Snoseeker：用于筛选人类基因组中的指南和孤儿snorna基因的高级计算套件。
影响因子：
14.9
作者：
Yang, Jian-Hua;Zhang, Xiao-Chen;Huang, Zhan-Peng;Zhou, Hui;Huang, Mian-Bo;Zhang, Shu;Chen, Yue-Qin;Qu, Liang-Hu
通讯作者：
Qu, Liang-Hu
影响因子：
56.9
作者：
Kapranov, Philipp;Cheng, Jill;Gingeras, Thomas R.
通讯作者：
Gingeras, Thomas R.
DOI：
10.1111/j.1432-1033.1987.tb13439.x
发表时间：
1987-10-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BOCHNIG, P;REUTER, R;LUHRMANN, R
通讯作者：
LUHRMANN, R
影响因子：
56.9
作者：
Morris, KV;Chan, SWL;Looney, DJ
通讯作者：
Looney, DJ
影响因子：
30.8
作者：
Ting, AH;Schuebel, KE;Baylin, SB
通讯作者：
Baylin, SB