Predicting human age with bloodstains by sjTREC quantification.
Predicting human age with bloodstains by sjTREC quantification.
复制标题
通过 sjTREC 量化根据血迹预测人类年龄
DOI：
10.1371/journal.pone.0042412
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sun HY
中科院分区：
文献类型：
--
作者：
Ou XL;Gao J;Wang H;Wang HS;Lu HL;Sun HY
我们先前的研究和其他报道表明，人外周血中信号联合T细胞受体重排切除环(SjTRECs)的年龄相关性下降。到目前为止，关于在血迹样本中检测sjTREC的研究只有很少的报道，这些研究是基于有限年龄范围的小样本样本，尽管血迹在法医实践中更常见。在这项研究中，我们采用灵敏的Taqman实时定量聚合酶链式反应(QPCR)方法对0-86岁(n = 264)个体的血痕进行sjTREC定量。结果表明，人血痕中sjTREC含量呈年龄依赖性下降(r = −0.8712)。年龄估算公式为年龄 = −7.1815Y−42.458±9.42(Y dCtTBP-sjTREC；9.42标准差)。此外，我们通过分析相同个体的新鲜和储存的血迹来测试短时间或长时间储存时间的影响。值得注意的是，在四周的储存时间内，新的和旧的DNA样本之间的sjTREC含量没有发现统计上的显著差异。然而，在31个样品中，储存1.5年后，sjTREC含量显著损失(0.16-1.93DCT)。此外，对长达20年的血痕进行的sjTREC初步定量表明，尽管在所有样本中都可以检测到sjTREC含量，并且sjTREC含量与献血者年龄高度相关，但相关系数r随时间而下降，这表明这种方法的预测准确性在年龄较大的样本中会恶化。我们的研究结果表明，sjTREC定量可能也适用于血痕中的年龄预测，未来对sjTREC定量的时间依赖性或其他潜在影响的研究可能会进一步提高预测精度。
The age-related decline of signal joint T-cell receptor rearrangement excision circles (sjTRECs) in human peripheral blood has been demonstrated in our previous study and other reports. Until now, only a few studies on sjTREC detection in bloodstain samples were reported, which were based on a small sample of subjects of a limited age range, although bloodstains are much more frequently encountered in forensic practice. In this present study, we adopted the sensitive Taqman real-time quantitative polymerase chain reaction (qPCR) method to perform sjTREC quantification in bloodstains from individuals ranging from 0–86 years old (n = 264). The results revealed that sjTREC contents in human bloodstains were declined in an age-dependent manner (r = −0.8712). The formula of age estimation was Age = −7.1815Y−42.458±9.42 (Y dCtTBP-sjTREC; 9.42 standard error). Furthermore, we tested for the influence of short- or long- storage time by analyzing fresh and stored bloodstains from the same individuals. Remarkably, no statistically significant difference in sjTREC contents was found between the fresh and old DNA samples over a 4-week of storage time. However, significant loss (0.16–1.93 dCt) in sjTREC contents was detected after 1.5 years of storage in 31 samples. Moreover, preliminary sjTREC quantification from up to 20-year-old bloodstains showed that though the sjTREC contents were detectable in all samples and highly correlated with donor age, a time-dependent decrease in the correlation coefficient r was found, suggesting the predicting accuracy of this described assay would be deteriorated in aged samples. Our findings show that sjTREC quantification might be also suitable for age prediction in bloodstains, and future researches into the time-dependent or other potential impacts on sjTREC quantification might allow further improvement of the predicting accuracy.
登录
查看更多内容
影响因子：
3.7
作者：
Bocklandt S;Lin W;Sehl ME;Sánchez FJ;Sinsheimer JS;Horvath S;Vilain E
通讯作者：
Vilain E
影响因子：
4.6
作者：
Pido-Lopez, J;Imami, N;Aspinall, R
通讯作者：
Aspinall, R
DOI：
10.1084/jem.190.5.725
发表时间：
1999-09-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Zhang L;Lewin SR;Markowitz M;Lin HH;Skulsky E;Karanicolas R;He Y;Jin X;Tuttleton S;Vesanen M;Spiegel H;Kost R;van Lunzen J;Stellbrink HJ;Wolinsky S;Borkowsky W;Palumbo P;Kostrikis LG;Ho DD
通讯作者：
Ho DD
影响因子：
--
作者：
Ferrando-Martinez, Sara;Franco, Jaime M.;Leal, Manuel
通讯作者：
Leal, Manuel
影响因子：
2.2
作者：
Tsuji, A;Ishiko, A;Ikeda, N
通讯作者：
Ikeda, N