Three-dimensional nanoscale molecular imaging by extreme ultraviolet laser ablation mass spectrometry.
Three-dimensional nanoscale molecular imaging by extreme ultraviolet laser ablation mass spectrometry.
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DOI：
10.1038/ncomms7944
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发表时间：
2015-04-23
影响因子：
16.6
通讯作者：
Menoni, Carmen S.
中科院分区：
文献类型：
--
作者：
Kuznetsov, Ilya;Filevich, Jorge;Dong, Feng;Woolston, Mark;Chao, Weilun;Anderson, Erik H.;Bernstein, Elliot R.;Crick, Dean C.;Rocca, Jorge J.;Menoni, Carmen S.
能够在纳米尺度上绘制分子组成图的分析探针在材料研究、生物学和医学中具有至关重要的作用。质谱学成像使可视化样品表面多个分子组分的空间组织成为可能。然而，对于质谱学成像来说，以亚微米分辨率绘制三维(3D)分子组成图是具有挑战性的。在这里，我们描述了一种质谱成像方法，该方法利用被吸收的极紫外光的高3D局部化及其与物质的根本不同的相互作用来从单个激光发射的小到50 zl的体积中确定分子组成。分子成像的横向分辨率为75 nm，深度分辨率为20 nm。这些结果提供了在纳米尺度上以3D形式可视化化学成分和化学变化的机会。质谱分析被用来绘制许多领域的材料和表面的组成图。在这里，作者报告了一种基于极紫外光激光消融的质谱学技术，除了提供高灵敏度的纳米级分辨率外，还可以对化学成分进行三维成像。
Analytical probes capable of mapping molecular composition at the nanoscale are of critical importance to materials research, biology and medicine. Mass spectral imaging makes it possible to visualize the spatial organization of multiple molecular components at a sample's surface. However, it is challenging for mass spectral imaging to map molecular composition in three dimensions (3D) with submicron resolution. Here we describe a mass spectral imaging method that exploits the high 3D localization of absorbed extreme ultraviolet laser light and its fundamentally distinct interaction with matter to determine molecular composition from a volume as small as 50 zl in a single laser shot. Molecular imaging with a lateral resolution of 75 nm and a depth resolution of 20 nm is demonstrated. These results open opportunities to visualize chemical composition and chemical changes in 3D at the nanoscale. Mass spectral analysis is used to map the composition of materials and surfaces in numerous fields. Here, the authors report a mass spectral technique based on extreme ultraviolet laser ablation that allows three-dimensional imaging of chemical composition in addition to giving highly sensitive nanoscale resolution.
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影响因子：
3.8
作者：
Heinbuch, S;Grisham, M;Rocca, JJ
通讯作者：
Rocca, JJ
影响因子：
7.4
作者：
Seeley, Erin H.;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.
DOI：
10.1073/pnas.0801374105
发表时间：
2008-11-25
影响因子：
11.1
作者：
Seeley, Erin H.;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.
影响因子：
2
作者：
Fletcher, John S.;Rabbani, Sadia;Vickerman, John C.
通讯作者：
Vickerman, John C.
影响因子：
11
作者：
Watrous, Jeramie D.;Phelan, Vanessa V.;Dorrestein, Pieter C.
通讯作者：
Dorrestein, Pieter C.