Tropism of Pandemic 2009 H1N1 Influenza a Virus.
Tropism of Pandemic 2009 H1N1 Influenza a Virus.
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DOI：
10.3389/fmicb.2012.00128
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发表时间：
2012
影响因子：
5.2
通讯作者：
Nakaya T
中科院分区：
文献类型：
--
作者：
Ramadhany R;Yasugi M;Nakamura S;Daidoji T;Watanabe Y;Takahashi K;Ikuta K;Nakaya T
2009年H1N1流感病毒（H1N1 pdm）血凝素（HA）基因受体结合位点的取代可能是决定病毒是否与人类或禽类受体结合的关键。先前的报告表明HA Gly 222和/或Arg 223允许病毒优先结合在禽类物种中发现的α 2，3-连接的唾液酸。我们还证明，在含胚鸡蛋中连续传代甲型流感病毒使病毒生长增加32至64倍，与HA蛋白中Gly 222或Arg 223的流行增加一致（Yasugi等人，）。在这项研究中，我们发现，α 2，3-linkage-tropic病毒在上呼吸道的次要基因型在通过鸡蛋传代后成为优势。在临床标本和鸡胚传代标本中均检测到具有HA的病毒，HA包含N125 D-Q223 R、N125 D-D187 E-Q223 R、K119 N-D222 G和K119 N-N129 S-D222 G。这些结果可能表明，蛋适应病毒，可能代表的α 2，3-linkage-tropic病毒，也存在于人类上呼吸道作为一个小群体，并在H1N1 pdm爆发期间在人类中传播。
Substitutions at the receptor-binding site of the pandemic H1N1 2009 influenza A virus (H1N1pdm) hemagglutinin (HA) gene may be critical in determining whether a virus binds to human or avian receptors. Previous reports suggest that HA Gly222 and/or Arg223 allow viruses to bind preferentially to the α2,3-linked sialic acid found in avian species. We also demonstrated that serial passaging of influenza A virus in embryonated chicken eggs increased viral growth 32- to 64-fold, coincident with the increased prevalence of Gly222 or Arg223 in HA protein (Yasugi et al.,). In this study, we showed that the minor genotype of α2,3-linkage-tropic viruses in upper airways became dominant after passaging through chicken eggs. Viruses possessing HA containing N125D-Q223R, N125D-D187E-Q223R, K119N-D222G, and K119N-N129S-D222G, were detected in both clinical specimens and egg-passaged samples. These results might suggest that egg-adapted viruses, likely represented by α2,3-linkage-tropic virus, were also present in human upper airways as a minor population and transmitted in humans during the outbreak of H1N1pdm.
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影响因子：
11.8
作者：
Nakamura S;Maeda N;Miron IM;Yoh M;Izutsu K;Kataoka C;Honda T;Yasunaga T;Nakaya T;Kawai J;Hayashizaki Y;Horii T;Iida T
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
Jin, Hong