An overview of gene regulation in bacteria by small RNAs derived from mRNA 3' ends.
An overview of gene regulation in bacteria by small RNAs derived from mRNA 3' ends.
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DOI：
10.1093/femsre/fuac017
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发表时间：
2022-09-02
影响因子：
11.3
通讯作者：
Vogel, Joerg
中科院分区：
文献类型：
--
作者：
Ponath, Falk;Hoer, Jens;Vogel, Joerg
在过去的二十年里，通过短碱基配对调节mRNA的小的非编码RNA（sRNA）已经从一种好奇心变成了细菌中主要的一类转录后调节因子。它们是许多应激反应和调节回路的组成部分，影响细菌生命的几乎所有方面。在21世纪初开创性的sRNA搜索之后，该领域迅速专注于细菌染色体基因间区域的保守sRNA基因。然而，很快就出现了细菌sRNA加工的mRNA 3′端片段的另一个丰富来源。一些这样的3′末端衍生的sRNA现在已经被表征，经常揭示出在不同的细胞过程中意想不到的保守功能。本文综述了这些3′端来源的sRNA的生物起源、分子机制、与Hfq或ProQ等RNA结合蛋白的相互作用以及它们的功能范围，从作为单个代谢基因的专门调节因子到在整体应激反应中构成整个非编码臂。最近的全球RNA相互作用组研究表明，功能性3′端衍生的sRNA的重要性被大大低估，并且这种类型的基因之间在mRNA水平上的交叉调节在细菌中比目前所认识的更普遍。有越来越多的细菌mRNAs的例子，产生小RNA切割从自己的3 '端转录后控制其他基因。
Over the past two decades, small noncoding RNAs (sRNAs) that regulate mRNAs by short base pairing have gone from a curiosity to a major class of post-transcriptional regulators in bacteria. They are integral to many stress responses and regulatory circuits, affecting almost all aspects of bacterial life. Following pioneering sRNA searches in the early 2000s, the field quickly focused on conserved sRNA genes in the intergenic regions of bacterial chromosomes. Yet, it soon emerged that there might be another rich source of bacterial sRNAs—processed 3′ end fragments of mRNAs. Several such 3′ end-derived sRNAs have now been characterized, often revealing unexpected, conserved functions in diverse cellular processes. Here, we review our current knowledge of these 3′ end-derived sRNAs—their biogenesis through ribonucleases, their molecular mechanisms, their interactions with RNA-binding proteins such as Hfq or ProQ and their functional scope, which ranges from acting as specialized regulators of single metabolic genes to constituting entire noncoding arms in global stress responses. Recent global RNA interactome studies suggest that the importance of functional 3′ end-derived sRNAs has been vastly underestimated and that this type of cross-regulation between genes at the mRNA level is more pervasive in bacteria than currently appreciated. There are a growing number of examples of bacterial mRNAs that produce small RNAs cleaved from their own 3′ ends to post-transcriptionally control other genes.
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影响因子：
6.4
作者：
Balasubramanian D;Ragunathan PT;Fei J;Vanderpool CK
通讯作者：
Vanderpool CK
DOI：
10.1126/science.aad9822
发表时间：
2016-04-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dar D;Shamir M;Mellin JR;Koutero M;Stern-Ginossar N;Cossart P;Sorek R
通讯作者：
Sorek R
影响因子：
3.6
作者：
Davis, Brigid M.;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.
影响因子：
3.6
作者：
Desgranges, Emma;Barrientos, Laura;Caldelari, Isabelle
通讯作者：
Caldelari, Isabelle
影响因子：
11.4
作者：
Bronesky, Delphine;Desgranges, Emma;Caldelari, Isabelle
通讯作者：
Caldelari, Isabelle