Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses.
Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses.
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氨氧化thaumarchaeota的时空动力学在不同的北极水质量中。
DOI：
10.3389/fmicb.2018.00024
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发表时间：
2018
影响因子：
5.2
通讯作者：
Øvreås L
中科院分区：
文献类型：
--
作者：
Müller O;Wilson B;Paulsen ML;Rumińska A;Armo HR;Bratbak G;Øvreås L
地球上最丰富的古菌群之一是Thaumarchaeota。它们被认为是海洋氨氧化的主要贡献者，这是氮的地球化学循环的关键步骤。它们的普遍成功归因于高度的基因组灵活性和生态位适应性。基于编码氨单加氧酶亚基A（amoA）的基因的差异，已经确定了在水柱中具有不同分布模式的两种不同生态型。我们使用16S rRNA基因的高通量测序结合古菌amoA功能基因克隆文库，以调查哪些环境因素正在推动Thaumarchaeota生态型在大西洋门户到北冰洋的分布，通过2014年的年度周期。我们观察到Thaumarchaeota丰度的特征垂直模式，全年在中层（>200米）水中具有高值，但在黑暗的冬季月份（1月，3月和11月）也在上层（<200米）水中具有高值。Thaumarchaeota群落由三个OTU占主导地位，平均占76% ± 11，相对丰度根据水体特征而不是深度或铵浓度而变化，如先前研究所示。不同OTU类型的丰度比与功能性amoA水簇类型的丰度比相似。总之，这表明在不同的水团内的生态类型的强选择，支持水团特征作为定义微生物群落结构的重要因素的一般想法。如果确实如本研究所建议的那样，Thaumarchaeota种群动态受一系列因素控制，这里描述为水体特征，而不仅仅是深度，那么水体流量的变化将不可避免地影响不同生态型的分布。
One of the most abundant archaeal groups on Earth is the Thaumarchaeota. They are recognized as major contributors to marine ammonia oxidation, a crucial step in the biogeochemical cycling of nitrogen. Their universal success is attributed to a high genomic flexibility and niche adaptability. Based on differences in the gene coding for ammonia monooxygenase subunit A (amoA), two different ecotypes with distinct distribution patterns in the water column have been identified. We used high-throughput sequencing of 16S rRNA genes combined with archaeal amoA functional gene clone libraries to investigate which environmental factors are driving the distribution of Thaumarchaeota ecotypes in the Atlantic gateway to the Arctic Ocean through an annual cycle in 2014. We observed the characteristic vertical pattern of Thaumarchaeota abundance with high values in the mesopelagic (>200 m) water throughout the entire year, but also in the epipelagic (<200 m) water during the dark winter months (January, March and November). The Thaumarchaeota community was dominated by three OTUs which on average comprised 76% ± 11 and varied in relative abundance according to water mass characteristics and not to depth or ammonium concentration, as suggested in previous studies. The ratios of the abundance of the different OTU types were similar to that of the functional amoA water cluster types. Together, this suggests a strong selection of ecotypes within different water masses, supporting the general idea of water mass characteristics as an important factor in defining microbial community structure. If indeed, as suggested in this study, Thaumarchaeota population dynamics are controlled by a set of factors, described here as water mass characteristics and not just depth alone, then changes in water mass flow will inevitably affect the distribution of the different ecotypes.
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影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
影响因子：
11
作者：
Abell, Guy C. J.;Revill, Andrew T.;Robert, Stanley S.
通讯作者：
Robert, Stanley S.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
DOI：
10.1073/pnas.0506625102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Francis, CA;Roberts, KJ;Oakley, BB
通讯作者：
Oakley, BB
影响因子：
11
作者：
Beman, J. Michael;Popp, Brian N.;Francis, Christopher A.
通讯作者：
Francis, Christopher A.