Conserved Function of ACYL-ACYL CARRIER PROTEIN DESATURASE 5 on Seed Oil and Oleic Acid Biosynthesis between Arabidopsis thaliana and Brassica napus.
Conserved Function of ACYL-ACYL CARRIER PROTEIN DESATURASE 5 on Seed Oil and Oleic Acid Biosynthesis between Arabidopsis thaliana and Brassica napus.
复制标题
酰基-酰基载体蛋白去饱和酶5对拟南芥和甘蓝型油菜种子油和油酸生物合成的保守功能
DOI：
10.3389/fpls.2017.01319
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发表时间：
2017
影响因子：
5.6
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Jin C;Li D;Gao C;Liu K;Qi S;Duan S;Li Z;Gong J;Wang J;Hai J;Chen M
先前的研究表明，拟南芥中的几个酰基-酰基载体蛋白脱饱和酶（AtAAD）成员负责油酸（C18：1）的生物合成。对另一个成员AtAAD 5及其在密切相关且具有商业重要性的植物甘蓝型油菜中的同源体BnAAD 5进行了有限的研究。在这里，我们发现AtAAD 5主要和专门在整个种子发育阶段的胚胎发育中表达。aad 5突变导致成熟种子中油脂和C18：1含量显著降低，硬脂酸（C18：0）含量显著增加，表明AtAAD 5在种子油脂生物合成中起着重要的促进作用。我们还从B的A3亚基因组中克隆了BnAAD 5 -1的全长编码序列。油菜自交系L111我们发现BnAAD 5 -1在A.拟南芥aad 5 -2突变体的含油量降低，C18：0和C18：1含量改变，与突变体的表型完全互补。这些结果有助于我们更好地了解AAD成员在A. thaliana和B.为B的遗传操作提供了一个有希望的靶点。油菜。
Previous studies have shown that several ACYL–ACYL CARRIER PROTEIN DESATURASE (AtAAD) members in Arabidopsis thaliana are responsible for oleic acid (C18:1) biosynthesis. Limited research has been conducted on another member, AtAAD5, and its paralog BnAAD5 in the closely related and commercially important plant, Brassica napus. Here, we found that AtAAD5 was predominantly and exclusively expressed in developing embryos at the whole seed developmental stages. The aad5 mutation caused a significant decrease in the amounts of oil and C18:1, and a considerable increase in the content of stearic acid (C18:0) in mature seeds, suggesting that AtAAD5 functioned as an important facilitator of seed oil biosynthesis. We also cloned the full-length coding sequence of BnAAD5-1 from the A3 subgenome of the B. napus inbred line L111. We showed that ectopic expression of BnAAD5-1 in the A. thaliana aad5-2 mutant fully complemented the phenotypes of the mutant, such as lower oil content and altered contents of C18:0 and C18:1. These results help us to better understand the functions of AAD members in A. thaliana and B. napus and provide a promising target for genetic manipulation of B. napus.
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影响因子：
5.1
作者：
Kachroo, Aardra;Shanklin, John;Kachroo, Pradeep
通讯作者：
Kachroo, Pradeep
DOI：
10.1073/pnas.1003530107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Le, Brandon H.;Cheng, Chen;Goldberg, Robert B.
通讯作者：
Goldberg, Robert B.
影响因子：
7.4
作者：
LIGHTNER, J;WU, JR;BROWSE, J
通讯作者：
BROWSE, J
MYB76 抑制拟南芥种子脂肪酸积累
影响因子：
5.6
作者：
Duan, Shaowei;Jin, Changyu;Chen, Mingxun
通讯作者：
Chen, Mingxun
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF