Temporal transitions in the post-mitotic nervous system of Caenorhabditis elegans.
Temporal transitions in the post-mitotic nervous system of Caenorhabditis elegans.
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DOI：
10.1038/s41586-021-04071-4
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发表时间：
2021-12
期刊：
影响因子：
64.8
通讯作者：
Hobert O
中科院分区：
文献类型：
--
作者：
Sun H;Hobert O
在大多数动物中，大部分神经系统在胚胎发育期间产生并组装成神经元回路。然而，在幼年阶段，神经系统仍然经历广泛的解剖学和功能变化，最终在成年阶段形成完全成熟的神经系统。有丝分裂后神经元在胚胎后发育过程中的分子变化以及控制这些时间转换的遗传程序还不清楚。利用模型系统C. elegans，我们全面表征了不同的功能状态（运动行为）和相应的不同分子状态（转录组）的有丝分裂后神经系统在胚后发育过程中的时间转换。我们观察到基因表达中普遍存在的神经元类型特异性变化，其中许多变化是由保守的异时miRNA lin-4的发育上调以及随后通过抑制其靶点转录因子lin-4来促进成熟神经元转录程序控制的。14。这些分子转换的功能相关性是由lin-14转录因子nlp-45（一种神经肽编码基因）的时间调节靶基因例证的，我们发现这是胚胎后发育过程中探索活动中几个不同时间转换所必需的。我们的研究为控制神经元类型特异性基因电池的调控策略提供了新的见解，以调节胚胎后发育的时间，性别和环境维度的不同行为状态。
In most animals, the majority of the nervous system is generated and assembled into neuronal circuits during embryonic development . However, during juvenile stages, nervous systems still undergo extensive anatomical and functional changes to eventually form a fully mature nervous system by the adult stage . The molecular changes in post-mitotic neurons across post-embryonic development and the genetic programs that control these temporal transitions are not well understood . Using the model system C. elegans, we comprehensively characterized the distinct functional states (locomotor behavior) and corresponding distinct molecular states (transcriptome) of the post-mitotic nervous system across temporal transitions during postembryonic development. We observed pervasive, neuron type-specific changes in gene expression, many of which are controlled by the developmental upregulation of the conserved heterochronic miRNA lin-4 and the subsequent promotion of a mature neuronal transcriptional program through the repression of its target, the transcription factor lin-14. The functional relevance of these molecular transitions are exemplified by a temporally regulated target gene of the lin-14 transcription factor, nlp-45, a neuropeptide-encoding gene, which we find to be required for several distinct temporal transitions in exploratory activity during postembryonic development. Our study provides new insights into regulatory strategies that control neuron-type specific gene batteries to modulate distinct behavioral states across temporal, sexual and environmental dimensions of post-embryonic development.
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DOI：
10.1073/pnas.0402680101
发表时间：
2004-05-25
影响因子：
11.1
作者：
Gogtay, N;Giedd, JN;Thompson, PM
通讯作者：
Thompson, PM
影响因子：
16.2
作者：
Cadwell, Cathryn R.;Bhaduri, Aparna;Mostajo-Radji, Mohammed A.;Keefe, Matthew G.;Nowakowski, Tomasz J.
通讯作者：
Nowakowski, Tomasz J.
影响因子：
--
作者：
Kumar N;Jain V;Singh A;Jagtap U;Verma S;Mukhopadhyay A
通讯作者：
Mukhopadhyay A
DOI：
10.1073/pnas.1312087110
发表时间：
2013-10-01
影响因子：
11.1
作者：
Berkseth, Matt;Ikegami, Kohta;Zarkower, David
通讯作者：
Zarkower, David
影响因子：
64.5
作者：
CHALFIE, M;HORVITZ, HR;SULSTON, JE
通讯作者：
SULSTON, JE