Epstein Barr virus and Helicobacter pylori co-infection are positively associated with severe gastritis in pediatric patients.
Epstein Barr virus and Helicobacter pylori co-infection are positively associated with severe gastritis in pediatric patients.
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DOI：
10.1371/journal.pone.0062850
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fuentes-Pananá EM
中科院分区：
文献类型：
--
作者：
Cárdenas-Mondragón MG;Carreón-Talavera R;Camorlinga-Ponce M;Gomez-Delgado A;Torres J;Fuentes-Pananá EM
幽门螺杆菌感染是儿童时期获得的，引起胃粘膜的慢性炎症反应，这被认为是以后生活中获得胃癌（GC）的主要危险因素。最近，eb病毒（EBV）感染也与胃癌有关。EBV在早期炎症反应中的作用及其与幽门螺杆菌感染的关系尚不清楚。在这里，我们评估了儿童EBV感染是否与胃炎的分期相关，以及与幽门螺杆菌的合并感染是否影响炎症的严重程度。对333例小儿慢性腹痛患者进行了研究。对他们进行胃活检，并根据悉尼系统对炎症进行分级；抽取外周血，检测血清中EBV抗体（抗vca抗体IgG和抗IgM抗体）和幽门螺杆菌抗体（抗全菌抗体IgG和抗caga抗体）。我们发现仅EBV感染的患儿表现为轻度单核（MN）和无多形核（PMN）浸润，而幽门螺杆菌感染的患儿表现为中度MN和轻度PMN浸润。相比之下，同时感染两种病原体的患者与严重胃炎显著相关。重要的是，与单独幽门螺杆菌CagA+感染相比，幽门螺杆菌CagA+/EBV+联合感染与严重MN （PR 3.0）和PMN （PR 7.2）细胞的相关性更强。小儿患者同时感染eb病毒和幽门螺杆菌与严重胃炎有关。即使单次感染幽门螺杆菌CagA+菌株也与轻度至中度浸润有关，这表明幽门螺杆菌和EBV在胃粘膜中的协同作用，并揭示了EBV在以前未被认识到的关键作用。这项研究指出，需要研究这两种病原体，以了解胃粘膜严重损伤背后的机制，这可以识别出在以后的生活中出现更严重病变的风险增加的儿童。
H. pylori infection is acquired during childhood and causes a chronic inflammatory response in the gastric mucosa, which is considered the main risk factor to acquire gastric cancer (GC) later in life. More recently, infection by Epstein-Barr virus (EBV) have also been associated with GC. The role of EBV in early inflammatory responses and its relationship with H. pylori infection remains poorly studied. Here, we assessed whether EBV infection in children correlated with the stage of gastritis and whether co-infection with H. pylori affected the severity of inflammation. 333 pediatric patients with chronic abdominal pain were studied. From them, gastric biopsies were taken and inflammation graded according to the Sydney system; peripheral blood was drawn and antibodies against EBV (IgG and IgM anti-VCA) and H. pylori (IgG anti-whole bacteria and anti-CagA) were measured in sera. We found that children infected only by EBV presented mild mononuclear (MN) and none polymorphonuclear (PMN) cell infiltration, while those infected by H. pylori presented moderate MN and mild PMN. In contrast, patients co-infected with both pathogens were significantly associated with severe gastritis. Importantly, co-infection of H. pylori CagA+/EBV+ had a stronger association with severe MN (PR 3.0) and PMN (PR 7.2) cells than cases with single H. pylori CagA+ infection. Co-infection with EBV and H. pylori in pediatric patients is associated with severe gastritis. Even single infections with H. pylori CagA+ strains are associated with mild to moderate infiltration arguing for a cooperative effect of H. pylori and EBV in the gastric mucosa and revealing a critical role for EBV previously un-appreciated. This study points out the need to study both pathogens to understand the mechanism behind severe damage of the gastric mucosa, which could identified children with increased risk to present more serious lesions later in life.
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影响因子：
8.8
作者：
Camargo, M. C.;Murphy, G.;Koriyama, C.;Pfeiffer, R. M.;Kim, W. H.;Herrera-Goepfert, R.;Corvalan, A. H.;Carrascal, E.;Abdirad, A.;Anwar, M.;Hao, Z.;Kattoor, J.;Yoshiwara-Wakabayashi, E.;Eizuru, Y.;Rabkin, C. S.;Akiba, S.
通讯作者：
Akiba, S.
影响因子：
3.1
作者：
Ryan, Julie L.;Shen, You-Jun;Morgan, Douglas R.;Thorne, Leigh B.;Kenney, Shannon C.;Dominguez, Ricardo L.;Gulley, Margaret L.
通讯作者：
Gulley, Margaret L.
影响因子：
11.1
作者：
Chen, Lih-Chyang;Wang, Li-Jie;Tsang, Nang-Ming;Ojcius, David M.;Chen, Chia-Chun;OuYang, Chun-Nan;Hsueh, Chuen;Liang, Ying;Chang, Kai-Ping;Chen, Chiu-Chin;Chang, Yu-Sun
通讯作者：
Chang, Yu-Sun
影响因子：
9.4
作者：
Fachiroh, J;Paramita, DK;Middeldorp, JM
通讯作者：
Middeldorp, JM
影响因子：
5.4
作者：
Baumann, M;Mischak, H;Hammerschmidt, W
通讯作者：
Hammerschmidt, W