Transforming growth factor-beta alters differentiation in cultures of avian neural crest-derived cells: effects on cell morphology, proliferation, fibronectin expression, and melanogenesis.
Transforming growth factor-beta alters differentiation in cultures of avian neural crest-derived cells: effects on cell morphology, proliferation, fibronectin expression, and melanogenesis.
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转化生长因子-β 改变禽类神经嵴衍生细胞培养物的分化：对细胞形态、增殖、纤连蛋白表达和黑素生成的影响。
DOI：
10.1016/0012-1606(92)90226-7
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发表时间：
1992
影响因子：
2.7
通讯作者：
McGuire,PG
中科院分区：
文献类型：
--
作者：
Rogers,SL;Gegick,PJ;Alexander,SM;McGuire,PG
Neural crest cell differentiation is responsive to a variety of extrinsic signals that include extracellular matrix (ECM) molecules and growth factors. Transforming growth factor-β (TGF-β) has diverse, cell type-specific effects, many of which involve regulation of synthesis of ECM molecules and their cell surface receptors. We are studying both separate and potentially interrelated influences of ECM and growth factors on crest differentiation and report here that TGF-β alters several aspects of crest cell behaviorin vitro. Clusters of quail neural crest cells were cultured in the presence and absence of 400 pMTGF-β1 and examined at 1, 3, and 5 days. When examined at 5 days, there was a dramatic decrease in the number of melanocytes in treated cultures, regardless of the onset or duration of TGF-β treatment. With continuous TGF-β treatment, or with treatment only during crest cluster formation on explanted neural tubes, many cells increased in area, becoming extremely flat. These changes were evident beginning on Day 3. While quantitative analyses of video images documented the size increase, several aspects of motility were relatively unchanged. Synthesis of fibronectin (FN) by approximately 11% of cells on Day 3 and 31% of cells on Day 5 was demonstrated by immunocytochemistry and was associated with a sixfold increase in FN mRNA by Day 5. Experiments which correlated FN immunoreactivity with incorporation of bromodeoxyuridine suggested that the population of large, flat, FN-positive cells did not proliferate selectively and that there was a slower rate of proliferation in TGF-β-treated cultures than in untreated cultures. The large FN-immunoreactive cells resemble cells derived from cepalic neural crest and raise interesting questions concerning potential roles for TGF-β in regulating crest differentiationin vivo.
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影响因子：
5.6
作者：
J. Plouët;D. Gospodarowicz
通讯作者：
J. Plouët;D. Gospodarowicz
对鸟类躯干神经嵴细胞迁移的时间和途径的活体染料分析。
DOI：
10.1242/dev.106.4.809
发表时间：
1989
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Serbedzija,GN;Bronner-Fraser,M;Fraser,SE
通讯作者：
Fraser,SE
碱性 FGF 和 TGF-β 1 影响禽类胚胎神经嵴衍生细胞的黑素生成。
DOI：
10.1242/dev.111.2.635
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Stocker,KM;Sherman,L;Rees,S;Ciment,G
通讯作者：
Ciment,G
异维A酸对体外神经嵴细胞行为的影响。
影响因子：
2.7
作者：
L. Smith;I. Lott;M. Bronner‐Fraser
通讯作者：
M. Bronner‐Fraser
影响因子：
4.6
作者：
R. Pelton;S. Nomura;H. Moses;B. Hogan
通讯作者：
R. Pelton;S. Nomura;H. Moses;B. Hogan