Next-generation sequencing of the soil nematode community enables the sustainability of banana plantations to be monitored.
Next-generation sequencing of the soil nematode community enables the sustainability of banana plantations to be monitored.
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DOI：
10.1016/j.apsoil.2021.103999
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Atkinson HJ
中科院分区：
文献类型：
--
作者：
Bell CA;Namaganda J;Urwin PE;Atkinson HJ
乌干达面临着相当大的挑战，以使其粮食生产与3%的年人口增长率相匹配。烹饪香蕉是该国生产最多的主要作物，但全国的年度收成并没有增加。这种作物生长在贫瘠的土壤上，这些土壤通常是有机施肥的，而且往往容易受到侵蚀。土壤线虫是土壤质量的生物指示物，可以支持环境监测和农业系统可持续性评估。这些无脊椎动物是可用分子方法的生物多样性的高度指标。因此，我们应用下一代土壤线虫DNA测序来评估乌干达香蕉种植园的土壤质量。目的是建立一种方法，用于构建环境生物安全档案的一个方面，未来的目标是评估转基因作物的影响和改善目前的种植系统。土壤样品没有显着差异，在任何测得的土壤化学因素，土壤质地或有机质的百分比。从土壤中回收的植物寄生虫以外的土壤线虫30个类群，支持9个香蕉种植园，再加上3个咖啡和香蕉-咖啡间作从东部和西部乌干达。聚类分析正确分配每个种植园的作物/间作种植时，丰富的类群，而不是类群的存在或不存在。这表明宿主对土壤中特定线虫物种的丰度有相当大的影响。总的来说，线虫更丰富的土壤从咖啡种植园比香蕉咖啡间作与最低的价值是从领域支持香蕉。只有基础和营养多样性指数和线虫的百分比是快速殖民者之间的三种种植园类型。所有15个人工林的土壤可以被归类为具有低物理干扰，有限的化学胁迫，中度高养分富集和平衡的分解通道的成熟的土壤网络条件。
Uganda faces a considerable challenge to match its food production to an annual population growth rate of 3%. Cooking bananas are the country's most produced staple crop but the annual national harvest is not increasing. The crop grows on infertile soils that are normally fertilised organically and often susceptible to erosion. Soil nematodes are well-established as bioindicators of soil quality that can support environmental monitoring and assessment of the sustainability of agricultural systems. These invertebrates are a highly ranked indicator of biodiversity with molecular approaches available. Consequently, we have applied next-generation DNA sequencing of soil nematodes to evaluate soil quality of Ugandan banana plantations. The aim is to establish a method for constructing an aspect of an environmental biosafety dossier with the future aim of assessing the impact of transgenic crops and improving current cropping systems. The soil samples did not differ significantly in any of the measured soil chemistry factors, soil texture or percentage of organic matter. Thirty taxons of soil nematodes other than the plant parasites were recovered from soil supporting nine banana plantations plus three each from coffee and banana-coffee interplants from East and West Uganda. Cluster analysis correctly allocated each plantation to the crop/intercrop being grown when based on the abundance of taxa rather than taxa presence or absence. This indicates that the host has considerable effects on the abundance of specific nematode species within the soil. Overall, nematodes were more abundant in soil from coffee plantations than from banana-coffee interplants with the lowest values being from fields supporting just banana. Only the basal and trophic diversity indices and the percentage of nematodes that are rapid colonisers varied between the three plantation types. The soil of all fifteen plantations can be classified as having a mature soil web condition with low physical disturbance, limited chemical stressors, moderately high nutrient enrichment and balanced decomposition channels.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
4.8
作者：
Ferris, H;Matute, MM
通讯作者：
Matute, MM
影响因子：
2.7
作者：
BONGERS, T
通讯作者：
BONGERS, T
影响因子：
4.8
作者：
Ferris, H;Bongers, T;de Goede, RGM
通讯作者：
de Goede, RGM
影响因子：
4.6
作者：
Boyce RM;Hathaway N;Fulton T;Reyes R;Matte M;Ntaro M;Mulogo E;Waltmann A;Bailey JA;Siedner MJ;Juliano JJ
通讯作者：
Juliano JJ