Discriminating patients with early-stage breast cancer from benign lesions by detection of oxidative DNA damage biomarker in urine.
Discriminating patients with early-stage breast cancer from benign lesions by detection of oxidative DNA damage biomarker in urine.
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通过检测尿液中氧化 DNA 损伤生物标志物区分早期乳腺癌患者和良性病变。
DOI：
10.18632/oncotarget.17831
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发表时间：
2017-08-08
期刊：
影响因子：
--
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Guo C;Li X;Ye M;Xu F;Yu J;Xie C;Cao X;Guo M;Yuan Y;Zheng S
乳腺癌是全世界妇女中最常见的诊断和死亡相关癌症之一。乳腺X线摄影通常用于筛查，对于筛查结果异常的患者，建议进行侵入性检查，如疼痛组织活检。然而，这些病例中有相当一部分是良性病变。因此，需要一种新的非侵入性方法来区分乳腺癌和良性病变。本研究采用高通量超高效液相色谱-电喷雾串联质谱（UPLC-ESI-MS/MS）技术，对60例早期乳腺癌患者（I、II期）、51例乳腺良性疾病患者和73例健康志愿者的尿液样本中的DNA氧化损伤标志物8-oxodG进行了检测。结果表明，早期乳腺癌患者尿8-oxodG浓度不仅显著高于健康对照组，而且显著高于乳腺良性疾病患者，而乳腺良性疾病组与健康对照组尿8-oxodG浓度无显著性差异。早期乳腺癌组与乳腺良性疾病组及健康对照组比较，差异有统计学意义。此外，还进行了Logistic回归分析和受试者工作特征（ROC）曲线分析。我们的研究结果表明，8-oxodG在尿液中的显着增加可能作为一个潜在的生物标志物的风险估计，早期筛查和检测乳腺癌，特别是用于区分早期乳腺癌良性病变。
Breast cancer is one of the most commonly diagnosed and death-related cancers in women worldwide. Mammography is routinely used for screening and invasive examinations such as painful tissue biopsies were recommended for patients with abnormal screening outcomes. However, a considerable proportion of these cases turn out to be benign lesions. Thus, novel non-invasive approach for discriminating breast cancer from benign lesions is desirable. Herein, we applied a high-throughput ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) analysis to determine the oxidative DNA damage biomarker, 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) in urine samples from 60 patients with early-stage breast cancer (stage I, II), 51 patients with benign breast diseases and 73 healthy volunteers. We demonstrated that the concentration of urinary 8-oxodG in patients with early-stage breast cancer was significantly higher not only than that in healthy controls, but also than that in patients with benign breast diseases, whereas no significant difference of urinary 8-oxodG level was observed between benign breast diseases group and healthy control group. Moreover, there was significant difference between early-stage breast cancer group and non-cancerous group which consisted of benign breast diseases patients and healthy controls. Besides, logistic regression analysis and receiver operator characteristic (ROC) curve analysis were also performed. Our findings indicate that the marked increase of 8-oxodG in urine may serve as a potential biomarker for the risk estimation, early screening and detection of breast cancer, particularly for discriminating early-stage breast cancer from benign lesions.
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影响因子：
4.6
作者：
Luan H;Liu LF;Tang Z;Zhang M;Chua KK;Song JX;Mok VC;Li M;Cai Z
通讯作者：
Cai Z
影响因子：
3.7
作者：
Beretov J;Wasinger VC;Millar EK;Schwartz P;Graham PH;Li Y
通讯作者：
Li Y
硫唑嘌呤和UVA光产生诱变的氧化DNA损伤。
DOI：
10.1126/science.1114233
发表时间：
2005-09-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
O'Donovan P;Perrett CM;Zhang X;Montaner B;Xu YZ;Harwood CA;McGregor JM;Walker SL;Hanaoka F;Karran P
通讯作者：
Karran P
影响因子：
2.7
作者：
Obtulowicz, Tomasz;Swoboda, Maja;Tudek, Barbara
通讯作者：
Tudek, Barbara
影响因子：
5
作者：
Burton, Casey;Dan, Yongbo;Bosnak, Cynthia P.
通讯作者：
Bosnak, Cynthia P.