Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota.
Resveratrol and its derivative pterostilbene ameliorate intestine injury in intrauterine growth-retarded weanling piglets by modulating redox status and gut microbiota.
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白藜芦醇及其衍生物紫檀芪通过调节氧化还原状态和肠道微生物群改善宫内生长迟缓的断奶仔猪的肠道损伤
DOI：
10.1186/s40104-021-00589-9
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发表时间：
2021-06-10
影响因子：
7
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
Chen Y;Zhang H;Chen Y;Jia P;Ji S;Zhang Y;Wang T
研究背景肠道疾病是导致胎儿宫内生长迟缓（IUGR）仔猪生长迟缓、发病率和死亡率高的重要因素。白藜芦醇（Resveratrol，RSV）及其衍生物紫檀芪（Pterostilbene，PT）是一类天然芪类化合物，具有抗氧化、抗炎等多种生物活性。本研究比较了RSV和PT对IUGR断奶仔猪肠道氧化还原状态和肠道菌群的保护作用。NBW仔猪接受基础日粮，而IUGR仔猪根据断奶时的体重分为三组，分别接受基础日粮和添加RSV的日粮（300 mg/kg），或补充PT的饲料结果与IUGR仔猪相比，RSV和PT均能改善IUGR引起的仔猪空肠绒毛高度下降，提高血浆二胺氧化酶活性和D-乳酸水平，并改善空肠细胞凋亡（P< 0.05）。RSV和PT通过促进核因子红细胞2相关因子2（Nrf 2）核转位，提高IUGR仔猪空肠SOD活性和SOD 2 mRNA及蛋白表达（P< 0.05）。与RSV相比，PT能显著提高IUGR仔猪空肠绒毛高度/隐窝深度比值和occludin mRNA及蛋白水平（P< 0.05）。PT在促进IUGR仔猪空肠Nrf 2核转位和抑制丙二醛积累方面也上级RSV（P< 0.05）。此外，RSV还能调节IUGR仔猪盲肠菌群的组成，表现为增加拟杆菌门、普雷沃氏菌属、粪杆菌属和副拟杆菌属的比例，抑制变形菌门、土杆菌属和放线杆菌属的生长（P< 0.05）。结论PT对IUGR仔猪肠道氧化应激的保护作用强于RSV，而RSV对肠道菌群的调节作用强于PT。
BackgroundIntestinal disorder is an important factor contributing to growth lag and high rates of morbidity and mortality of piglets with intrauterine growth retardation (IUGR). Resveratrol (RSV) and its derivative pterostilbene (PT) are natural stilbenes possessing various bioactivities, such as antioxidative and anti-inflammatory effects. This study compared the protective potential of RSV and PT on the intestinal redox status and gut microbiota in weanling piglets with IUGR.MethodsEighteen male piglets of normal body weight (NBW) and 54 same-sex IUGR piglets were chosen according to their birth and weaning weights. The NBW piglets accepted a basal diet, while the IUGR piglets were allotted to one of three groups according to their body weight at weaning and received a basal diet, an RSV-supplemented diet (300 mg/kg), or a PT-supplemented diet (300 mg/kg), respectively.ResultsCompared with IUGR piglets, both RSV and PT improved the IUGR-associated decrease in jejunal villus height and increases in plasma diamine oxidase activity and D-lactate level and jejunal apoptosis of piglets (P< 0.05). Administering RSV and PT also enhanced jejunal superoxide dismutase activity and the mRNA and protein expression of superoxide dismutase 2 of IUGR piglets by promoting nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation (P< 0.05). Comparatively, PT was more effective than RSV in elevating the villus height/crypt depth ratio and occludin mRNA and protein levels in the jejunum of IUGR piglets (P< 0.05). PT was also superior to RSV in increasing Nrf2 nuclear translocation and inhibiting malondialdehyde accumulation in the jejunum of IUGR piglets (P< 0.05). Additionally, RSV modulated the composition of cecal microbiota of IUGR piglets, as evidenced by increasing the prevalence of the phylum Bacteroidetes and the generaPrevotella,Faecalibacterium,andParabacteroidesand inhibiting the growth of the phylum Proteobacteria and its generaEscherichiaandActinobacillus(P< 0.05). Moreover, RSV significantly increased the butyrate concentration in the cecum of IUGR piglets (P< 0.05).ConclusionPT is more potent than RSV to prevent intestinal oxidative stress, while RSV has a stronger capacity to regulate gut microbiota compared to PT.
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影响因子：
3.5
作者：
Bhakkiyalakshmi, Elango;Dineshkumar, Kesavan;Ramkumar, Kunka Mohanram
通讯作者：
Ramkumar, Kunka Mohanram
影响因子：
6.1
作者：
Chiou, Yi-Siou;Tsai, Mei-Ling;Pan, Min-Hsiung
通讯作者：
Pan, Min-Hsiung
影响因子：
4
作者：
Mikstacka, Renata;Rimando, Agnes M.;Ignatowicz, Ewa
通讯作者：
Ignatowicz, Ewa
DOI：
10.1097/mpg.0b013e3181d75d52
发表时间：
2010-10-01
影响因子：
2.9
作者：
Fanca-Berthon, Pascale;Hoebler, Christine;Michel, Catherine
通讯作者：
Michel, Catherine
影响因子：
1.8
作者：
Lin, Hai-Shu;Yue, Bing-De;Ho, Paul C.
通讯作者：
Ho, Paul C.