Ultrathin calcinated films on a gold surface for highly effective laser desorption/ionization of biomolecules.
Ultrathin calcinated films on a gold surface for highly effective laser desorption/ionization of biomolecules.
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DOI：
10.1021/ac100132x
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发表时间：
2010-06-15
影响因子：
7.4
通讯作者：
Cheng Q
中科院分区：
文献类型：
--
作者：
Duan J;Linman MJ;Cheng Q
我们报告了在金底物上制造的纳米级钙化硅酸盐膜，用于生物分子的高效，无基质激光解吸电离质谱（LDI-MS）分析。钙化的膜是由逐层（LBL）沉积/钙化过程制备的，其中硅酸盐层的厚度及其表面特性得到精确控制。该膜在低质量区域的LDI-MS中表现出出色的效率，可有效分析低质量样品并检测包括氨基酸，肽和蛋白质在内的大型生物分子。蓄电膜的其他优点包括易于制备和修改，高可重现性，低成本和出色的可重复性。已系统地研究了影响LDI对钙化膜的实验参数。样品中柠檬酸的存在通过促进分析物的质子化并减少碎片化，从而显着提高了LDI的性能。润湿特性和表面粗糙度似乎是操纵分析物的LDI性能的重要因素。这种新的底物在无基质质谱方法的发展中呈现出明显的进步，并且非常适合分析具有高灵敏度的广泛质量范围内的生物分子。它可能为与现有的微流体和光学方法集成在一起时开发新的途径，以开发新的技术平台。
We report a nanoscale calcinated silicate film fabricated on a gold substrate for highly effective, matrix-free laser desorption ionization mass spectrometry (LDI-MS) analysis of biomolecules. The calcinated film is prepared by a layer-by-layer (LbL) deposition/calcination process wherein the thickness of the silicate layer and its surface properties are precisely controlled. The film exhibits outstanding efficiency in LDI-MS with extremely low background noise in the low-mass region, allowing for effective analysis of low mass samples and detection of large biomolecules including amino acids, peptides and proteins. Additional advantages for the calcinated film include ease of preparation and modification, high reproducibility, low cost and excellent reusability. Experimental parameters that influence LDI on calcinated films have been systemically investigated. Presence of citric acid in the sample significantly enhances LDI performance by facilitating protonation of the analyte and reducing fragmentation. The wetting property and surface roughness appear to be important factors that manipulate LDI performance of the analytes. This new substrate presents a marked advance in the development of matrix-free mass spectrometric methods and is uniquely suited for analysis of biomolecules over a broad mass range with high sensitivity. It may open new avenues for developing novel technology platforms upon integration with existing methods in microfluidics and optics.
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影响因子：
3.5
作者：
DeRosa, RL;Schader, PA;Shelby, JE
通讯作者：
Shelby, JE
影响因子：
4.4
作者：
Alimpiev, S.;Grechnikov, A.;Nikiforov, S.
通讯作者：
Nikiforov, S.
影响因子：
4.4
作者：
Alimpiev, S;Nikiforov, S;Sunner, J
通讯作者：
Sunner, J
影响因子：
7.4
作者：
Kruse, RA;Li, XL;Sweedler, JV
通讯作者：
Sweedler, JV
影响因子：
7.4
作者：
Okuno, S;Arakawa, R;Wada, Y
通讯作者：
Wada, Y