Biodegradation of Methyl tert-Butyl Ether by Co-Metabolism with a Pseudomonas sp. Strain.

Biodegradation of Methyl tert-Butyl Ether by Co-Metabolism with a Pseudomonas sp. Strain.
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与假单胞菌菌株共代谢的甲基叔丁基醚的生物降解

DOI:
10.3390/ijerph13090883
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发表时间:
2016-09-06
影响因子:
--
通讯作者:
Yan W
Yan W
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Li S;Wang S;Yan W

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甲基叔丁基醚(MTBE)是近年来在世界范围内发现的一种常见的地下水污染物,共代谢生物修复技术被认为是消除该污染物的一种有前途的方法。本论文从一株具有降解甲基叔丁基醚(MTBE)能力的细菌中分离得到一株菌株DZ 13,经16 S rRNA基因测序分析,将其命名为假单胞菌(Pseudomonassp.DZ13)。菌株DZ 13能在正构烷烃(C5-C8)上生长,并伴随着MTBE的共代谢降解。不同碳数的正构烷烃对MTBE的降解速率和叔丁醇的积累有显著影响。以正戊烷为生长基质,假单胞菌DZ 13共代谢降解MTBE时,MTBE的降解速率较高(Vmax = 38.1nmol/min/mgprotein,Ks = 6.8mmol/L),TBA的积累较低。在连续降解实验中,假单胞菌DZ 13对MTBE的去除率在连续投加5次后没有明显下降。
Co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (MTBE), which was found to be a common pollutant worldwide in the ground or underground water in recent years. In this paper, bacterial strain DZ13 (which can co-metabolically degrade MTBE) was isolated and named as Pseudomonas sp. DZ13 based on the result of 16S rRNA gene sequencing analysis. Strain DZ13 could grow on n-alkanes (C5-C8), accompanied with the co-metabolic degradation of MTBE. Diverse n-alkanes with different carbon number showed a significant influence on the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA). When Pseudomonas sp. DZ13 co-metabolically degraded MTBE with n-pentane as the growth substrate, a higher MTBE-degrading rate (Vmax = 38.1 nmol/min/mgprotein, Ks = 6.8 mmol/L) and lower TBA-accumulation was observed. In the continuous degradation experiment, the removal efficiency of MTBE by Pseudomonas sp. Strain DZ13 did not show an obvious decrease after five times of continuous addition.
DOI: 10.1111/j.1574-6941.2010.00847.x
发表时间: 2010-05-01
影响因子: 4.2
作者:
Malandain, Cedric;Fayolle-Guichard, Francoise;Vogel, Timothy M.
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期刊: Reviews in Environmental Science and Bio/Technology
影响因子: --
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