Increased apoptosis rate of human decidual cells and cytotrophoblasts in patients with recurrent spontaneous abortion as a result of abnormal expression of CDKN1A and Bax.
Increased apoptosis rate of human decidual cells and cytotrophoblasts in patients with recurrent spontaneous abortion as a result of abnormal expression of CDKN1A and Bax.
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DOI：
10.3892/etm.2016.3692
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发表时间：
2016-11
影响因子：
2.7
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Lv X;Cai Z;Li S
本研究分析了复发性流产患者滋养细胞和人蜕膜细胞的增殖和凋亡情况及其相关的细胞通路机制。选取34例复发性流产患者和30例计划性人工流产的健康孕妇。通过负压抽吸技术收集滋养层和蜕膜细胞。采用TUNEL法检测细胞凋亡率。采用免疫组化方法检测TP53蛋白。采用实时定量PCR检测CDKN1A和Bax mRNA的相对表达水平。结果发现，反复流产组细胞凋亡率显着升高，TP53蛋白、CDKN1A、Bax mRNA表达水平也显着升高（p<0.05）。综上所述，反复流产患者的滋养层细胞和蜕膜细胞均出现明显的凋亡，这可能与TP53蛋白通过细胞途径介导的CDKN1A和Bax基因的异常表达有关。
In the present study, we analyzed the proliferation and apoptosis of trophoblasts and human decidual cells in patients with recurrent spontaneous abortion and the related cellular pathway mechanism. Thirty-four patients with recurrent abortion and 30 healthy pregnant women undergoing planned artificial abortion were selected. The trophoblast and decidual cells were collected by negative pressure aspiration technique. TUNEL method was used to detect the apoptosis rate. Immunohistochemical method was used for detection of TP53 protein. Quantitative real-time PCR was used for detection of the relative expression level of CDKN1A and Bax mRNA. It was found that the cell apoptosis rate in the recurrent miscarriage group was significantly increased and the expression levels of TP53 protein, CDKN1A and Bax mRNA were also significantly increased (p<0.05). In conclusion, the trophoblast and decidual cells of patients with recurrent abortion were obviously apoptotic, which was probably related to abnormal expression of the CDKN1A and Bax genes mediated by TP53 protein through cellular pathways.
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