Antibody evasion of SARS-CoV-2 Omicron BA.1, BA.1.1, BA.2, and BA.3 sub-lineages.
Antibody evasion of SARS-CoV-2 Omicron BA.1, BA.1.1, BA.2, and BA.3 sub-lineages.
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DOI：
10.1016/j.chom.2022.05.001
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发表时间：
2022-08-10
影响因子：
30.3
通讯作者：
Wang, Pengfei
中科院分区：
文献类型：
--
作者：
Ai, Jingwen;Wang, Xun;He, Xinyi;Zhao, Xiaoyu;Zhang, Yi;Jiang, Yuchao;Li, Minghui;Cui, Yuchen;Chen, Yanjia;Qiao, Rui;Li, Lin;Yang, Lulu;Li, Yi;Hu, Zixin;Zhang, Wenhong;Wang, Pengfei
SARS-CoV-2 Omicron变异体已经进化成四个亚系-BA.1，BA.1.1，BA.2和BA.3-其中BA.2在全球范围内占主导地位。我们和其他人已经报道了BA.1和BA.2的抗体逃避，但Omicron亚系与疫苗引起的或单克隆抗体（mAb）介导的中和的并排比较是必要的。使用基于VSV的假病毒，我们报告了来自接种两剂灭活全病毒体疫苗的个体的血清显示出弱至无中和活性，而同源或异源加强剂显著提高了针对所有Omicron亚谱系的中和滴度。我们还针对Omicron亚系提供了20种mAb组（包括10种授权或批准的mAb）的中和谱，沿着的是针对单一或组合尖峰突变的mAb图谱。大多数mAb失去了中和活性，而一些mAb在Omicron亚系中表现出不同的中和模式，反映了抗原性差异。总的来说，我们的研究结果表明，Omicron亚系威胁到当前疫苗和抗体治疗的中和效力，突出了疫苗增强剂的重要性。Ai等人报告了Omicron BA.1、BA.1.1、BA.2和BA.3亚系对BBIBP-CorV疫苗引起的中和的并行敏感性特征，发现对两剂疫苗接种者血清的大量逃避，但不是加强的。单个和组合突变分析绘制了Omicron Spike的关键残基沿着图，这些残基有助于避免单克隆抗体介导的中和。
The SARS-CoV-2 Omicron variant has evolved into four sub-lineages—BA.1, BA.1.1, BA.2, and BA.3—with BA.2 becoming dominant worldwide. We and others have reported antibody evasion of BA.1 and BA.2, but side-by-side comparisons of Omicron sub-lineages to vaccine-elicited or monoclonal antibody (mAb)-mediated neutralization are necessary. Using VSV-based pseudovirus, we report that sera from individuals vaccinated by two doses of an inactivated whole-virion vaccine shows weak to no neutralization activity, while homologous or heterologous boosters markedly improve neutralization titers against all Omicron sub-lineages. We also present neutralization profiles against a 20 mAb panel, including 10 authorized or approved, against the Omicron sub-lineages, along with mAb mapping against single or combinatorial spike mutations. Most mAbs lost neutralizing activity, while some demonstrate distinct neutralization patterns among Omicron sub-lineages, reflecting antigenic differences. Collectively, our results suggest the Omicron sub-lineages threaten the neutralization efficacy of current vaccines and antibody therapeutics, highlighting the importance of vaccine boosters. Ai et al. report side-by-side susceptibility profiles of Omicron BA.1, BA.1.1, BA.2, and BA.3 sub-lineages to BBIBP-CorV vaccine-elicited neutralization, finding substantial evasion against two-dose, but not boosted, vaccinee sera. Single and combinatorial mutational analysis maps key residues along Omicron Spike that help facilitate evasion against monoclonal antibody-mediated neutralization.
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影响因子：
13.2
作者：
Ai J;Zhang H;Zhang Y;Lin K;Zhang Y;Wu J;Wan Y;Huang Y;Song J;Fu Z;Wang H;Guo J;Jiang N;Fan M;Zhou Y;Zhao Y;Zhang Q;Liu Q;Lv J;Li P;Qiu C;Zhang W
通讯作者：
Zhang W
影响因子：
16.6
作者：
Kim C;Ryu DK;Lee J;Kim YI;Seo JM;Kim YG;Jeong JH;Kim M;Kim JI;Kim P;Bae JS;Shim EY;Lee MS;Kim MS;Noh H;Park GS;Park JS;Son D;An Y;Lee JN;Kwon KS;Lee JY;Lee H;Yang JS;Kim KC;Kim SS;Woo HM;Kim JW;Park MS;Yu KM;Kim SM;Kim EH;Park SJ;Jeong ST;Yu CH;Song Y;Gu SH;Oh H;Koo BS;Hong JJ;Ryu CM;Park WB;Oh MD;Choi YK;Lee SY
通讯作者：
Lee SY
影响因子：
17.1
作者：
Jones BE;Brown-Augsburger PL;Corbett KS;Westendorf K;Davies J;Cujec TP;Wiethoff CM;Blackbourne JL;Heinz BA;Foster D;Higgs RE;Balasubramaniam D;Wang L;Zhang Y;Yang ES;Bidshahri R;Kraft L;Hwang Y;Žentelis S;Jepson KR;Goya R;Smith MA;Collins DW;Hinshaw SJ;Tycho SA;Pellacani D;Xiang P;Muthuraman K;Sobhanifar S;Piper MH;Triana FJ;Hendle J;Pustilnik A;Adams AC;Berens SJ;Baric RS;Martinez DR;Cross RW;Geisbert TW;Borisevich V;Abiona O;Belli HM;de Vries M;Mohamed A;Dittmann M;Samanovic MI;Mulligan MJ;Goldsmith JA;Hsieh CL;Johnson NV;Wrapp D;McLellan JS;Barnhart BC;Graham BS;Mascola JR;Hansen CL;Falconer E
通讯作者：
Falconer E
影响因子：
30.3
作者：
Tauzin A;Nayrac M;Benlarbi M;Gong SY;Gasser R;Beaudoin-Bussières G;Brassard N;Laumaea A;Vézina D;Prévost J;Anand SP;Bourassa C;Gendron-Lepage G;Medjahed H;Goyette G;Niessl J;Tastet O;Gokool L;Morrisseau C;Arlotto P;Stamatatos L;McGuire AT;Larochelle C;Uchil P;Lu M;Mothes W;De Serres G;Moreira S;Roger M;Richard J;Martel-Laferrière V;Duerr R;Tremblay C;Kaufmann DE;Finzi A
通讯作者：
Finzi A
影响因子：
64.5
作者：
Garcia-Beltran WF;St Denis KJ;Hoelzemer A;Lam EC;Nitido AD;Sheehan ML;Berrios C;Ofoman O;Chang CC;Hauser BM;Feldman J;Roederer AL;Gregory DJ;Poznansky MC;Schmidt AG;Iafrate AJ;Naranbhai V;Balazs AB
通讯作者：
Balazs AB