Perilla Oil Supplementation Ameliorates High-Fat/High-Cholesterol Diet Induced Nonalcoholic Fatty Liver Disease in Rats via Enhanced Fecal Cholesterol and Bile Acid Excretion.
Perilla Oil Supplementation Ameliorates High-Fat/High-Cholesterol Diet Induced Nonalcoholic Fatty Liver Disease in Rats via Enhanced Fecal Cholesterol and Bile Acid Excretion.
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补充紫苏油通过增强粪便胆固醇和胆汁酸排泄来改善高脂肪/高胆固醇饮食引起的大鼠非酒精性脂肪肝
DOI：
10.1155/2016/2384561
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发表时间：
2016
影响因子：
--
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Chen T;Yuan F;Wang H;Tian Y;He L;Shao Y;Li N;Liu Z
最近的实验研究和临床试验表明，肝脏胆固醇代谢紊乱与非酒精性脂肪性肝病(NAFLD)的发生密切相关。本研究的主要目的是研究富含α-亚麻酸(ALA)的紫苏油对NASH的抑制作用，并深入了解其可能的作用机制。大鼠在高脂/高胆固醇饮食(HFD)中添加紫苏油(POH)，连续16周。常规血液生化检测和组织学染色结果表明，紫苏油能改善高脂血症大鼠的高脂血症，减轻肝脏脂肪变性，抑制肝脏炎性细胞浸润和纤维化。紫苏油还增加了粪便胆汁酸和胆固醇的排泄。肝脏RNA-Seq分析发现，长期补充紫苏油显著改变了胆固醇代谢相关基因的表达。我们的结果表明，长期喂饲高水平的胆固醇后，大鼠肝脏内源性合成胆固醇和富含胆固醇的低密度脂蛋白的摄取受到显著抑制，紫苏油并不调节胆固醇合成相关基因的表达，但确实增加了通过转化为胆汁酸从肝细胞中清除的胆固醇，并增加了粪便中胆固醇的排泄。
Recent experimental studies and clinical trials have shown that hepatic cholesterol metabolic disorders are closely related to the development of nonalcoholic fatty liver disease (NAFLD). The main goal of this study was to investigate the efficacy of the perilla oil rich in alpha-linolenic acid (ALA) against NASH and gain a deep insight into its potential mechanisms. Rats were fed a high-fat/high-cholesterol diet (HFD) supplement with perilla oil (POH) for 16 weeks. Routine blood biochemical tests and histological staining illustrated that the perilla oil administration improved HFD-induced hyperlipidemia, reduced hepatic steatosis, and inhibited hepatic inflammatory infiltration and fibrosis. Perilla oil also increased fecal bile acid and cholesterol excretion. Hepatic RNA-Seq analysis found that the long time perilla oil supplement notably modified the gene expression involved in cholesterol metabolism. Our results implicate that, after long-term high level dietary cholesterol feeding, rat liver endogenous synthesis of cholesterol and cholesterol-rich low density lipoprotein uptake was significantly inhibited, and perilla oil did not modulate expression of genes responsible for cholesterol synthesis but did increase cholesterol removed from hepatocytes by conversion to bile acids and increased fecal cholesterol excretion.
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影响因子：
2.8
作者：
Alvarez JA;Ashraf A
通讯作者：
Ashraf A
影响因子：
25.7
作者：
Allard, Johane P.;Aghdassi, Elaheh;Heathcote, Jenny E.
通讯作者：
Heathcote, Jenny E.
影响因子：
6
作者：
Araya, J;Rodrigo, R;Poniachik, J
通讯作者：
Poniachik, J
影响因子：
29
作者：
Min HK;Kapoor A;Fuchs M;Mirshahi F;Zhou H;Maher J;Kellum J;Warnick R;Contos MJ;Sanyal AJ
通讯作者：
Sanyal AJ
影响因子：
13.5
作者：
Kleiner, DE;Brunt, EM;Sanyal, AJ
通讯作者：
Sanyal, AJ