Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans.
Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans.
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DOI：
10.3390/molecules26196080
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发表时间：
2021-10-08
期刊：
影响因子：
--
通讯作者：
Harding SE
中科院分区：
文献类型：
--
作者：
Harding SE
Analytical ultracentrifugation is a versatile approach for analysing the molecular mass, molecular integrity (degradation/aggregation), oligomeric state and association/dissociation constants for self-association, and assay of ligand binding of kinase related membrane proteins and glycans. It has the great property of being matrix free—providing separation and analysis of macromolecular species without the need of a separation matrix or membrane or immobilisation onto a surface. This short review—designed for the non-hydrodynamic expert—examines the potential of modern sedimentation velocity and sedimentation equilibrium and the challenges posed for these molecules particularly those which have significant cytoplasmic or extracellular domains in addition to the transmembrane region. These different regions can generate different optimal requirements in terms of choice of the appropriate solvent (aqueous/detergent). We compare how analytical ultracentrifugation has contributed to our understanding of two kinase related cellular or bacterial protein/glycan systems (i) the membrane erythrocyte band 3 protein system—studied in aqueous and detergent based solvent systems—and (ii) what it has contributed so far to our understanding of the enterococcal VanS, the glycan ligand vancomycin and interactions of vancomycin with mucins from the gastrointestinal tract.
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影响因子：
48
作者：
Chayen, Naomi E.;Saridakis, Emmanuel
通讯作者：
Saridakis, Emmanuel
DOI：
10.1007/s00249-018-1321-3
发表时间：
2018-10
期刊：
European biophysics journal : EBJ
影响因子：
--
作者：
Harding SE
通讯作者：
Harding SE
影响因子：
2
作者：
Colfen, H;Harding, SE
通讯作者：
Harding, SE
影响因子：
4.6
作者：
Chaptal V;Delolme F;Kilburg A;Magnard S;Montigny C;Picard M;Prier C;Monticelli L;Bornert O;Agez M;Ravaud S;Orelle C;Wagner R;Jawhari A;Broutin I;Pebay-Peyroula E;Jault JM;Kaback HR;le Maire M;Falson P
通讯作者：
Falson P
影响因子：
3.4
作者：
Dam, J;Schuck, P
通讯作者：
Schuck, P