Genome-wide identification and functional analysis of the TIFY gene family in the response to multiple stresses in Brassica napus L.
Genome-wide identification and functional analysis of the TIFY gene family in the response to multiple stresses in Brassica napus L.
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DOI：
10.1186/s12864-020-07128-2
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发表时间：
2020-10-22
期刊：
影响因子：
4.4
通讯作者：
Hua W
中科院分区：
文献类型：
--
作者：
He X;Kang Y;Li W;Liu W;Xie P;Liao L;Huang L;Yao M;Qian L;Liu Z;Guan C;Guan M;Hua W
TIFY是一个植物特异性蛋白家族，在植物发育和对胁迫和激素的反应中具有多种功能，包括JASMONATE zm -domain （JAZ）、TIFY、PPD和ZML亚家族。尽管在许多其他物种中对TIFY家族进行了广泛的研究，但在甘蓝型油菜中尚未发现TIFY的特征。在本研究中，我们分别在甘蓝型油菜（B. napus）、油菜（B. rapa）和甘蓝（B.甘蓝）基因组中鉴定了77、36和39个TIFY家族基因。系统发育分析结果表明，拟南芥、甘蓝型油菜、油菜和甘蓝中的170个TIFY蛋白可分为11个类群：7个JAZ类群、1个PPD类群、1个TIFY类群和2个ZIM/ZML类群。分子进化分析表明，TIFY基因在芸苔科植物中具有保守性。基因表达谱和qRT-PCR显示，不同组的BnaTIFY成员在正常条件下或在不同的非生物/生物胁迫和激素处理下具有不同的时空表达模式。BnaJAZ亚家族基因主要在根中表达，在叶片中受到NaCl、PEG、冷冻、茉莉酸甲酯（MeJA）、水杨酸（SA）和核核菌的上调，表明该亚家族基因在调控甘蓝型油菜多重胁迫耐受的激素信号中起着重要作用。BnaTIFY基因的大量注释和表达分析有助于我们了解这些基因在甘蓝型油菜多重胁迫反应和植物激素串扰中的功能。
TIFY is a plant-specific protein family with a diversity of functions in plant development and responses to stress and hormones, which contains JASMONATE ZIM-domain (JAZ), TIFY, PPD and ZML subfamilies. Despite extensive studies of TIFY family in many other species, TIFY has not yet been characterized in Brassica napus. In this study, we identified 77, 36 and 39 TIFY family genes in the genome of B. napus, B. rapa and B. oleracea, respectively. Results of the phylogenetic analysis indicated the 170 TIFY proteins from Arabidopsis, B. napus, B. rapa and B. oleracea could be divided into 11 groups: seven JAZ groups, one PPD group, one TIFY group, and two ZIM/ZML groups. The molecular evolutionary analysis showed that TIFY genes were conserved in Brassicaceae species. Gene expression profiling and qRT-PCR revealed that different groups of BnaTIFY members have distinct spatiotemporal expression patterns in normal conditions or following treatment with different abiotic/biotic stresses and hormones. The BnaJAZ subfamily genes were predominantly expressed in roots and up-regulated by NaCl, PEG, freezing, methyl jasmonate (MeJA), salicylic acid (SA) and Sclerotinia sclerotiorum in leaves, suggesting that they have a vital role in hormone signaling to regulate multiple stress tolerance in B. napus. The extensive annotation and expression analysis of the BnaTIFY genes contributes to our understanding of the functions of these genes in multiple stress responses and phytohormone crosstalk in B. napus.
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影响因子：
3.5
作者：
Guo, Xiaomei;Stotz, Henrik U.
通讯作者：
Stotz, Henrik U.
DOI：
10.1073/pnas.1811828115
发表时间：
2018-11-06
影响因子：
11.1
作者：
Guo Q;Yoshida Y;Major IT;Wang K;Sugimoto K;Kapali G;Havko NE;Benning C;Howe GA
通讯作者：
Howe GA
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影响因子：
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作者：
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通讯作者：
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影响因子：
11.6
作者：
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通讯作者：
Howe, Gregg A.
影响因子：
10.7
作者：
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通讯作者：
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