Enhancing the Long-Term Stability of a Polymer Dot Glucose Transducer by Using an Enzymatic Cascade Reaction System.
Enhancing the Long-Term Stability of a Polymer Dot Glucose Transducer by Using an Enzymatic Cascade Reaction System.
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利用酶级联反应系统增强聚合物点葡萄糖传感器的长期稳定性
DOI：
10.1002/adhm.202001019
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发表时间：
2021-03
影响因子：
10
通讯作者：
Chiu DT
中科院分区：
文献类型：
--
作者：
Sun K;Ding Z;Zhang J;Chen H;Qin Y;Xu S;Wu C;Yu J;Chiu DT
糖尿病患者的糖代谢受损会导致严重的急性和长期并发症，这使得血糖的实时检测对于糖尿病患者来说是必不可少的。现有的连续葡萄糖监测系统由于长期稳定性差而不适合长期临床血糖管理。聚合物点（Pdot）葡萄糖传感器是可植入的光学纳米传感器，具有出色的亮度，灵敏度，选择性和生物相容性。在这里，我们表明，过氧化氢-Pdot葡萄糖传感器中的葡萄糖氧化的产物-通过光漂白降低传感器性能，降低葡萄糖氧化酶活性，并产生细胞毒性。通过将过氧化氢酶添加到基于葡萄糖氧化酶的Pdot传感器中以产生酶级联，葡萄糖氧化的过氧化氢产物被过氧化氢酶快速分解，防止其积累并改善传感器的光稳定性、酶活性和生物相容性。因此，我们提出了下一代Pdot葡萄糖传感器与多酶反应系统（Pdot-GOx/CAT），提供了出色的传感特性以及更大的检测系统稳定性。Pdot葡萄糖传感器结合了这种酶级联以消除过氧化氢，将具有更高的长期稳定性，以改善糖尿病患者的连续葡萄糖监测。过氧化氢作为Pdot葡萄糖传感器中葡萄糖氧化的产物，通过光漂白降低传感器性能，降低葡萄糖氧化酶活性，并产生细胞毒性。通过引入由葡萄糖氧化酶和过氧化氢酶组成的仿生酶级联，在葡萄糖氧化过程中产生的过氧化氢被快速分解，大大提高了Pdot葡萄糖传感器的传感特性和检测系统的稳定性。
Impaired glucose metabolism in diabetes causes severe acute and long-term complications, making real-time detection of blood glucose indispensable for diabetic patients. Existing continuous glucose monitoring systems are unsuitable for long-term clinical glycemic management due to poor long-term stability. Polymer dot (Pdot) glucose transducers are implantable optical nanosensors that exhibit excellent brightness, sensitivity, selectivity, and biocompatibility. Here we show that hydrogen peroxide—a product of glucose oxidation in Pdot glucose sensors—degraded sensor performance via photobleaching, reduced glucose oxidase activity, and generated cytotoxicity. By adding catalase to a glucose oxidase-based Pdot sensor to create an enzymatic cascade, the hydrogen peroxide product of glucose oxidation is rapidly decomposed by catalase, preventing its accumulation and improving the sensor’s photostability, enzymatic activity, and biocompatibility. Thus, we present a next-generation Pdot glucose transducer with a multienzyme reaction system (Pdot-GOx/CAT) that provides excellent sensing characteristics as well as greater detection system stability. Pdot glucose transducers that incorporate this enzymatic cascade to eliminate hydrogen peroxide will possess greater long-term stability for improved continuous glucose monitoring in diabetic patients. Hydrogen peroxide, as a product of glucose oxidation in Pdot glucose sensors, degraded sensor performance via photobleaching, reduced glucose oxidase activity, and generated cytotoxicity. By incorporating a biomimetic enzymatic cascade consisting of glucose oxidase and catalase, hydrogen peroxide produced during glucose oxidation is rapidly decomposed, greatly improving sensing characteristics and detection system stability of the Pdot glucose transducer.
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影响因子：
9
作者：
通讯作者：
--
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
62.1
作者：
Nichols, Scott P.;Koh, Ahyeon;Storm, Wesley L.;Shin, Jae Ho;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
影响因子：
5.1
作者：
Ogurtsova, K.;Fernandes, J. D. da Rocha;Makaroff, L. E.
通讯作者：
Makaroff, L. E.
纳米颗粒酶通过过氧化氢的产生和光解增强光疗
影响因子：
10.8
作者：
Chang, Kaiwen;Liu, Zhihe;Wu, Changfeng
通讯作者：
Wu, Changfeng