Phagotrophic Protist Diversity in the Groundwater of a Karstified Aquifer – Morphological and Molecular Analysis
Phagotrophic Protist Diversity in the Groundwater of a Karstified Aquifer – Morphological and Molecular Analysis
复制标题
岩溶含水层地下水中吞噬原生生物的多样性——形态和分子分析
DOI：
10.1111/jeu.12054
复制
发表时间：
2013
影响因子：
2.2
通讯作者：
Küsel
中科院分区：
文献类型：
--
作者：
Risse-Buhl;Herrmann;Pizani;Schönborn;Totsche;K. U. ;Küsel
为了阐明地下水中微生物食物网的结构，了解原生生物的多样性和摄食策略是必不可少的。我们应用培养依赖的方法和分子方法进一步了解地下水中的原生生物多样性。地下水取样来自图林根盆地（图林根，德国）的岩溶含水层。可培养原生生物丰度估计高达8，000个细胞/L。在地下水富集培养物中检测到11种鞭毛虫、10种裸阿米巴和1种纤毛虫形态种。大多数鞭毛虫形态种，通常< 10 μm，是无柄或自由游泳的悬浮饲养者，例如，Spumellaspp.，Monosigaspp.，和移动的表面相关形式，其抓住生物膜，例如，波多属通常< 35 μm的裸阿米巴，其抓取生物膜的代表为，例如，Vahlkampfiaspp.，Vannellaspp.，和Hartmanellaspp. 18 S rRNA基因序列的最大部分隶属于Spumella-like Stratifiles。此外，在地下水克隆库中还检测到与真菌和后生食草动物相关的序列。我们假设小型原生生物物种在岩溶含水层的裂缝和裂隙的结构化表面避难，主要以生物膜相关或悬浮细菌为食。
To clarify the structure of microbial food webs in groundwater, knowledge about the protist diversity and feeding strategies is essential. We applied cultivation‐dependent approaches and molecular methods for further understanding of protist diversity in groundwater. Groundwater was sampled from a karstified aquifer located in the Thuringian Basin (Thuringia, Germany). Cultivable protist abundance estimated up to 8,000 cells/L. Eleven flagellates, 10 naked amoebae, and one ciliate morpho‐species were detected in groundwater enrichment cultures. Most of the flagellates morpho‐species, typically < 10 μm, were sessile or free swimming suspension feeders, e.g.,Spumellaspp.,Monosigaspp., and mobile, surface‐associated forms that grasp biofilms, e.g.,Bodospp. Naked amoebae, typically < 35 μm, that grasp biofilms were represented by, e.g.,Vahlkampfiaspp.,Vannellaspp., andHartmanellaspp. The largest fraction of the 18S rRNA gene sequences was affiliated withSpumella‐like Stramenopiles. Besides, also sequences affiliated with fungi and metazoan grazers were detected in clone libraries of the groundwater. We hypothesize that small sized protist species take refuge in the structured surface of the fractures and fissures of the karstified aquifer and mainly feed on biofilm‐associated or suspended bacteria.
登录
查看更多内容
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
2.6
作者：
Buonanno, Federico;Guella, Graziano;Ortenzi, Claudio
通讯作者：
Ortenzi, Claudio
影响因子：
5.1
作者：
Finlay, Bland J.;Esteban, Genoveva F.
通讯作者：
Esteban, Genoveva F.
影响因子：
2.2
作者：
BUTLER, H;ROGERSON, A
通讯作者：
ROGERSON, A
DOI：
10.1016/s0003-9365(89)80015-6
发表时间：
1989-01-01
期刊：
ARCHIV FUR PROTISTENKUNDE
影响因子：
--
作者：
FENCHEL, T;FINLAY, BJ;GIANNI, A
通讯作者：
GIANNI, A