Molecular immunophenotyping of lungs in naïve and vaccinated chickens early after pulmonary avian influenza A (H9N2) virus infection
Molecular immunophenotyping of lungs in naïve and vaccinated chickens early after pulmonary avian influenza A (H9N2) virus infection
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甲型禽流感 (H9N2) 病毒感染后早期对未接种疫苗和接种疫苗的鸡进行肺部分子免疫表型分析
DOI：
10.1016/j.vetimm.2008.10.245
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发表时间：
2009
影响因子：
1.8
通讯作者：
Degen W
中科院分区：
文献类型：
--
作者：
Degen W
在呼吸道感染模型中，我们研究了低致病性禽流感病毒A H9 N2株感染鸡的免疫反应。对于分子免疫应答分析，我们采用了最近开发的一种含有约5000个cDNA元件的鸡免疫微阵列，一式两份（Smith等人，2006 J. Smith，D. Speed，PM Hocking，RT塔尔博特，WG Degen，VE Schijns，EJ Glass和DW Burt，鸡5 K微阵列靶向免疫功能的开发，BMC Genomics 7（2006），第49页。全文通过CrossRef|查看Scopus中的记录|引用自Scopus（0）Smith等人，2006年）。在第一个实验中，对肉鸡进行模拟免疫（称为非免疫）、仅用灭活病毒抗原接种（免疫）或用不同Th 1或Th 2极化免疫增强剂中的病毒抗原接种（免疫增强）。接种后三周，所有动物通过眼-鼻-鼻内途径进行H9 N2呼吸道感染。从这些动物中取出肺和脾，分离RNA并随后用于微阵列分析。在一般情况下，我们注意到较少的宿主基因表达免疫增强，即佐剂，鸟类相比，非免疫，感染鸡。免疫增强的鸟类表现出降低的先天反应，主要限于热休克蛋白，这可能足以控制肺部感染与诱导的抗体和T细胞。微阵列数据的评估表明不同免疫组中特有或共有的基因途径（Degen et al.，2006年）。在随后的实验中，我们分析了，并仍然是分析，年龄和类型（肉鸡与蛋鸡）的鸡，攻击途径（眼-鼻-肠道与喷雾），和使用其他免疫反应极化免疫增强剂对宿主基因表达谱的影响，通过使用微阵列和定量RT-PCR（Q-RT-PCR），以及经典的免疫参数。在本演示中，我们将重点介绍我们的发现。在这种天然甲型流感病毒靶物种（即鸡）中收集的数据对于设计含有免疫应答修饰免疫增强剂的未来人类大流行和季节性疫苗具有重要意义。
In a respiratory infection model we study immune reactions in chickens infected with the low pathogenic avian influenza A H9N2 virus strain. For molecular immune response profiling we employed a recently developed chicken immuno-microarray containing approximately 5000 cDNA elements in duplicate (Smith et al., 2006 J. Smith, D. Speed, PM Hocking, RT Talbot, WG Degen, VE Schijns, EJ Glass and DW Burt, Development of a chicken 5 K microarray targeted towards immune function, BMC Genomics 7 (2006), p. 49. Full Text via CrossRef| View Record in Scopus| Cited By in Scopus (0) Smith et al., 2006). In a first experiment, broiler-type chickens were either mock-immunized (referred to as non-immune), vaccinated with inactivated viral antigen only (immune), or with viral antigen in distinct Th1 or Th2 polarizing immunopotentiators (immune potentiated). Three weeks after vaccination all animals were given a respiratory infection with H9N2 via the oculo-nasal-intratracheal route. From these animals lungs and spleens were removed, RNA was isolated and subsequently used for microarray analysis. In general, we noted less host gene expression in immune potentiated, ie adjuvanted, birds when compared to non-immune, infected chickens. Immune potentiated birds showed reduced innate responses, mainly restricted to heat shock proteins, which, likely, are sufficient to control the pulmonary infection together with induced antibodies and T cells. Evaluation of the microarray data suggested gene pathways unique to or common amongst the differently immune groups (Degen et al., 2006). In subsequent experiments we have analyzed, and still are analyzing, the influence of age and type (broiler versus layer) of the chickens, challenge route (oculo-nasal-intratracheal versus spray), and the use of other immune response polarizing immunopotentiators on the host gene expression profiles by using the microarray and quantitative RT-PCR (Q-RT-PCR), as well as classical immune parameters. In this presentation we will highlight our findings. The data collected in this natural influenza A virus target Species, ie chicken, are of interest for the design of future human pandemic and seasonal vaccines containing immune response modifying immunopotentiators.
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仅使用抗原进行鼻肺免疫后，肺部环境会促进 Th2 细胞反应，但在强烈免疫刺激的情况下会诱导 Th1 细胞反应1
影响因子：
4.4
作者：
H. Jones;Lisa Hodge;K. Fujihashi;H. Kiyono;J. Mcghee;J. Simecka
通讯作者：
J. Simecka
DOI：
10.1073/pnas.160270697
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, YP;Shaw, M;Hay, A
通讯作者：
Hay, A
DOI：
10.1046/j.1432-1033.2002.03252.x
发表时间：
2002-11-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Nathanson, CM;Wassélius, J;Abrahamson, M
通讯作者：
Abrahamson, M
影响因子：
3.8
作者：
Liu, BX;Mori, I;Kimura, Y
通讯作者：
Kimura, Y
爱丁堡研究探索者开发针对免疫功能的鸡 5 K 微阵列
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
S. Bollen
通讯作者：
S. Bollen