Ecological niche modelling and nDNA sequencing support a new, morphologically cryptic beetle species unveiled by DNA barcoding.
Ecological niche modelling and nDNA sequencing support a new, morphologically cryptic beetle species unveiled by DNA barcoding.
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DOI：
10.1371/journal.pone.0016662
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发表时间：
2011-02-09
期刊：
影响因子：
3.7
通讯作者：
Balke M
中科院分区：
文献类型：
--
作者：
Hawlitschek O;Porch N;Hendrich L;Balke M
用于估计生物多样性的 DNA 测序技术（例如 DNA 条形码）可能会揭示神秘物种。然而，条形码和形态数据之间的分歧已经引起了争议。因此，物种界定不应仅基于线粒体DNA。在这里，我们探索了 nDNA 和生物气候模型在 mtDNA 序列数据揭示的一种新水生甲虫物种中的应用。澳大利亚水生甲虫动物群的特点是高度特有性，包括本地辐射，例如安提孔甲虫属。 Antiporus femoralis 以前被认为存在于澳大利亚西南部和东南部两个分离但形态上无法区分的种群中。我们构建了安提波鲁斯的系统发育学，并发现这些种群之间存在深刻的分歧。编码精氨酸激酶基因的高度可变核蛋白的诊断特征证实了两个分离群体的存在。然后，我们使用生态位模型来检查两个种群的气候生态位特征。所有结果都支持这两个种群作为不同物种的地位。我们将西南物种描述为 Antiporus occidentalis sp.n。 除了 nDNA 序列数据和线粒体序列的扩展使用之外，生态位模型在描述形态上神秘的物种方面具有巨大的潜力。
DNA sequencing techniques used to estimate biodiversity, such as DNA barcoding, may reveal cryptic species. However, disagreements between barcoding and morphological data have already led to controversy. Species delimitation should therefore not be based on mtDNA alone. Here, we explore the use of nDNA and bioclimatic modelling in a new species of aquatic beetle revealed by mtDNA sequence data. The aquatic beetle fauna of Australia is characterised by high degrees of endemism, including local radiations such as the genus Antiporus. Antiporus femoralis was previously considered to exist in two disjunct, but morphologically indistinguishable populations in south-western and south-eastern Australia. We constructed a phylogeny of Antiporus and detected a deep split between these populations. Diagnostic characters from the highly variable nuclear protein encoding arginine kinase gene confirmed the presence of two isolated populations. We then used ecological niche modelling to examine the climatic niche characteristics of the two populations. All results support the status of the two populations as distinct species. We describe the south-western species as Antiporus occidentalis sp.n. In addition to nDNA sequence data and extended use of mitochondrial sequences, ecological niche modelling has great potential for delineating morphologically cryptic species.
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影响因子：
6.5
作者：
De Queiroz, Kevin
通讯作者：
De Queiroz, Kevin
DOI：
10.1098/rspb.2009.0015
发表时间：
2009-07-07
影响因子：
4.7
作者：
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通讯作者：
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影响因子：
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作者：
Balke, Michael;Pons, Joan;Vogler, Alfried P.
通讯作者：
Vogler, Alfried P.