Gender- and stressor-specific microRNA expression in Tribolium castaneum.
Gender- and stressor-specific microRNA expression in Tribolium castaneum.
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DOI：
10.1098/rsbl.2012.0273
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发表时间：
2012-10-23
期刊：
影响因子：
3.3
通讯作者：
Vilcinskas A
中科院分区：
文献类型：
--
作者：
Freitak D;Knorr E;Vogel H;Vilcinskas A
微小RNA（microRNAs，miRNAs）是真核生物中一类非编码的小分子RNA，参与基因表达的转录后调控。为了解决它们在调节昆虫对环境胁迫的生理适应中的作用，我们选择赤拟谷盗作为模型。给甲虫喂食细菌性昆虫病原体嗜虫假单胞菌（模拟自然感染），注射肽聚糖（强免疫反应的实验设置）或进行轻度热休克或饥饿。使用实时PCR定量所选免疫和应激相关基因的差异表达，并使用专有微阵列确定455种成熟节肢动物miRNA的表达和诱导。我们发现，赤拟谷盗表现出性别和应激特异性的免疫基因和miRNA表达的调整。引人注目的是，我们发现女性中应激诱导的miRNAs数量明显高于男性。这一观察结果可以支持称为贝特曼免疫原理的假设，该原理预测了性别特异性免疫反应，因为女性通过增加寿命获得健康，而男性通过增加交配率获得健康。我们的研究结果表明，赤拟谷盗男性和女性显示不同的调节元件，转录前和转录后，可能导致不同的投资策略的生活史特征。
MicroRNAs (miRNAs) are small non-coding RNAs mediating post-transcriptional regulation of gene expression in eukaryotes. Addressing their role in regulation of physiological adaptations to environmental stress in insects, we selected the red flour beetle Tribolium castaneum as a model. Beetles were fed with the bacterial entomopathogen Pseudomonas entomophila (to mimic natural infection), injected with peptidoglycan (experimental setting of strong immune responses) or subjected to either mild heat shock or starvation. Differential expression of selected immunity- and stress-related genes was quantified using real-time PCR, and expression and induction of 455 mature arthropod miRNAs were determined using proprietary microarrays. We found that Tribolium exhibits both gender- and stressor-specific adjustment of immune gene and miRNA expression. Strikingly, we discovered that the number of stressor-induced miRNAs in females is remarkably higher than in males. This observation could support the hypothesis called Bateman's principle in immunity that predicts gender-specific immune responses because females gain fitness through increased longevity, whereas males gain fitness by increasing mating rates. Our results suggest that Tribolium males and females display differential regulatory elements, both pre- and post-transcriptional, likely resulting from different investment strategies in life-history traits.
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影响因子：
3.7
作者：
Knorr E;Schmidtberg H;Vilcinskas A;Altincicek B
通讯作者：
Altincicek B
DOI：
10.1098/rstb.2008.0148
发表时间：
2009-01-12
影响因子：
6.3
作者：
Nunn, Charles L.;Lindenfors, Patrik;Rolff, Jens
通讯作者：
Rolff, Jens
影响因子：
64.8
作者：
Becker, Thomas;Loch, Gerrit;Hoch, Michael
通讯作者：
Hoch, Michael
影响因子：
2.9
作者：
Altincicek, Boran;Knorr, Eileen;Vilcinskas, Andreas
通讯作者：
Vilcinskas, Andreas
影响因子：
2.4
作者：
Knorr, Eileen;Vilcinskas, Andreas
通讯作者：
Vilcinskas, Andreas