BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum.
BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum.
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BAG9 通过调节细胞氧化还原稳态和热激蛋白的稳定性赋予番茄耐热性
DOI：
10.3390/antiox11081467
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发表时间：
2022-07-27
期刊：
影响因子：
7
通讯作者：
Zhou, Jie
中科院分区：
文献类型：
--
作者：
Huang, Huamin;Liu, Chenxu;Yang, Chen;Kanwar, Mukesh Kumar;Shao, Shujun;Qi, Zhenyu;Zhou, Jie
Bcl-2-associated athanogene（BAG）家族是一组在植物群和动物群中共享保守结构域的共分子伴侣，参与植物的生长、发育和抗逆性。然而，番茄BAG基因在耐热性中的功能在很大程度上仍然未知。在此，我们发现BAG 9的表达在番茄植物中的热胁迫期间被诱导。通过CRISPR/Cas9敲除BAG 9基因减少，而其过表达增加番茄植物的耐热性，如表型、光合速率和膜过氧化反应所反映的。热诱导的活性氧和氧化/氧化蛋白质进一步增加bag 9突变体和BAG 9过表达植物正常化。此外，抗氧化酶，抗坏血酸（阿萨）/脱氢抗坏血酸（DHA），还原型谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）的活动减少在bag 9突变体和BAG 9过表达植物在热胁迫下增加。此外，BAG 9在体外和体内与Hsp 20蛋白相互作用。热诱导的热休克蛋白的积累表现出减少bag 9突变体，同时，它增加了BAG 9过表达的植物。因此，BAG 9通过调节细胞氧化还原稳态和热休克蛋白的稳定性在热应激反应中起着至关重要的作用。
The Bcl-2-associated athanogene (BAG) family, a group of co-chaperones that share conservative domains in flora and fauna, is involved in plant growth, development, and stress tolerance. However, the function of tomato BAG genes on thermotolerance remains largely unknown. Herein, we found that the expression of BAG9 was induced during heat stress in tomato plants. Knockout of the BAG9 gene by CRISPR/Cas9 reduced, while its overexpression increased thermotolerance in tomato plants as reflected by the phenotype, photosynthesis rate, and membrane peroxidation. Heat-induced reactive oxygen species and oxidative/oxidized proteins were further increased in bag9 mutants and were normalized in BAG9 overexpressing plants. Furthermore, the activities of antioxidant enzymes, ascorbic acid (AsA)/dehydroascorbic acid (DHA), and reduced glutathione (GSH)/oxidized glutathione (GSSG) were reduced in bag9 mutants and were increased in BAG9 overexpressing plants under heat stress. Additionally, BAG9 interacted with Hsp20 proteins in vitro and in vivo. Accumulation of Hsp proteins induced by heat showed a reduction in bag9 mutants; meanwhile, it was increased in BAG9 overexpressing plants. Thus, BAG9 played a crucial role in response to heat stress by regulating cellular redox homeostasis and the stability of heat shock proteins.
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影响因子：
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作者：
Cheng F;Yin LL;Zhou J;Xia XJ;Shi K;Yu JQ;Zhou YH;Foyer CH
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