Circulating miR-21 serves as a serum biomarker for hepatocellular carcinoma and correlated with distant metastasis.
Circulating miR-21 serves as a serum biomarker for hepatocellular carcinoma and correlated with distant metastasis.
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循环 miR-21 作为肝细胞癌的血清生物标志物并与远处转移相关
DOI：
10.18632/oncotarget.17211
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发表时间：
2017-07-04
期刊：
影响因子：
--
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Guo X;Lv X;Lv X;Ma Y;Chen L;Chen Y
血清miRNAs作为几种癌症的循环生物标志物已经得到了广泛的应用。在这项研究中，我们的目的是评估血清miR-21作为肝细胞癌（HCC）患者和其他对照的新生物标志物的诊断效率。共招募了533人，并进行了两步分析。试验组包括40名HCC患者和40名健康供体。原发性肝癌组织中miR-21的表达水平显著高于癌旁组织（P<0.0001）。HCC患者血清miR-21水平显著高于HD患者（P<0.0001）。在验证组中，175例HCC患者的平均血清miR-21水平明显高于64例CHB、78例LC和136例HD（均P<0.0001）。ROC曲线显示miR-21的AUC为0.849，敏感性为82.1%，特异性为83.9%。此外，血清miR-21在AFP阴性HCC亚组中保持其诊断效率，AUC为0.831，灵敏度为81.2%，特异性为83.2%。血清miR-21水平可区分HCC、CHB和LC（AUC分别为0.789和0.814，灵敏度分别为76.9%和85.7%; AUC分别为80.8%和72.9%）。此外，血清miR-21水平与临床分期（P=0.006）和远处转移（P=0.000）显著相关。因此，我们的研究结果表明，miR-21与AFP可能有助于提高HCC的诊断，特别是AFP阴性HCC，并可以区分HCC与CHB和LC。
Serum miRNAs have gained great popularity to act as circulating biomarkers of several cancers. In this study, we aimed to evaluate the diagnostic efficiency of serum miR-21 as novel biomarkers for patients with hepatocellular carcinoma (HCC) and other controls. A total of 533 individuals were recruited and conducted in a two-step analysis. The pilot group included 40 HCC patients and 40 healthy donors. The expression levels of miR-21 were significantly higher in primary HCC tissues than in adjacent noncancerous tissues (P<0.0001). HCC patients exhibited significantly higher serum levels of miR-21 than HD (P<0.0001). In the verification group, the mean serum levels of miR-21 in 175 patients with HCC were significantly higher than in 64 with CHB, 78 with LC and 136 HD (all P<0.0001). ROC curves demonstrated that the AUC of miR-21 was 0.849, sensitivity 82.1% and specificity 83.9%. Furthermore, serum miR-21 maintained its diagnostic efficiency in AFP-negative HCC subgroups with AUC 0.831, sensitivity 81.2% and specificity 83.2%. The serum levels of miR-21 could distinguish HCC from CHB and LC (AUC 0.789, sensitivity 76.9%, specificity 85.7% and AUC 0.814, sensitivity 80.8%, specificity 72.9%, respectively). In addition, the serum levels of miR-21 were significantly associated with clinical stage (P=0.006) and distant metastasis (P=0.000). Thus, our findings suggest that miR-21 together with AFP may help enhance the diagnosis of HCC, especially of AFP-negative HCC, and could distinguish HCC from CHB and LC.
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