Orientation of the haems of the ubiquinol oxidase:O2 reductase, cytochrome bo of Escherichia coli.

Orientation of the haems of the ubiquinol oxidase:O2 reductase, cytochrome bo of Escherichia coli.
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泛醇氧化酶:O2 还原酶、大肠杆菌细胞色素 bo 的血红素方向。

DOI:
10.1111/j.1432-1033.1991.tb16082.x
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发表时间:
1991
期刊:
European journal of biochemistry
影响因子:
--
通讯作者:
Ingledew,WJ
Ingledew,WJ
中科院分区:
--
文献类型:
--
作者:
Salerno,JC;Ingledew,WJ

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利用电子顺磁共振技术对细胞质膜片段的定向多层制备进行了研究,确定了大肠杆菌大红泛醇氧化酶o2还原酶的两个血红蛋白的取向。该酶含有一个低纺丝样血红素和一个高纺丝样血红素,分别被命名为细胞色素带。这两种血红蛋白的表面都垂直于膜平面,进一步扩展了这种酶与哺乳动物细胞色素氧化酶cytochromeaa3在结构和功能上的相似性。
The orientation of the two haems of theEscherichia coliubiquinol oxidase:O2reductase, cytochromebo, has been determined by electron paramagnetic resonance studies on oriented multilayer preparations of cytoplasmic membrane fragments. The enzyme contains a low‐spinb‐like haem and a high‐spinb‐like haem, designated cytochromesbandorespectively. Both haems are oriented with their planes perpendicular to the membrane plane, further extending the catalogue of structural and functional similarities between this enzyme and the mammalian cytochromecoxidase, cytochromeaa3.
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