In vivo and in vitro inhibition of cyp19 gene expression by prostaglandin F2alpha in murine luteal cells: implication of GATA-4.
In vivo and in vitro inhibition of cyp19 gene expression by prostaglandin F2alpha in murine luteal cells: implication of GATA-4.
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小鼠黄体细胞中前列腺素 F2α 对 cyp19 基因表达的体内和体外抑制：GATA-4 的影响。
作者：
C. Stocco
A major function of the corpus luteum (CL) is to secrete progesterone. In rats, this gland also produces significant amounts of 17beta-estradiol. Progesterone and 17beta-estradiol are important regulators of rat luteal cell function. Estrogen biosynthesis is catalyzed by P450aromatase (P450arom), which is encoded by the cyp19 gene. In the rat CL, P450arom is expressed throughout pregnancy until the day before parturition, when it rapidly decreases. The mechanisms that control P450arom expression in luteal cells, particularly, the one or more factors that cause its rapid fall before parturition, are not known. Inasmuch as prostaglandin (PG) F(2alpha) plays a key role in the regulation of luteal function at the end of pregnancy, the purpose of this investigation was to determine whether PGF(2alpha) affect the expression of P450arom in the CL before parturition. PGF(2alpha) decreased luteal P450arom mRNA and protein levels in vivo and in vitro. A decrease in P450arom mRNA was also observed in mice CL just before parturition, but this change did not take place in PGF(2alpha) receptor knockout mice. The time course of the decrease in P450arom mRNA by PGF(2alpha) reflected the P450arom mRNA half-life determined by actinomycin D. Moreover, nuclear run-on assay showed that PGF(2alpha) attenuates P450arom gene transcription. Gel shift assays revealed that GATA-4 binds to the P450aromatase promoter, and that such binding is increased by PGF(2alpha). It is concluded that PGF(2alpha) decreases luteal P450arom mRNA levels at the end of pregnancy in rodents by inhibiting cyp19 expression.
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影响因子：
4.8
作者：
Ying Zhao;V. Agarwal;C. Mendelson;E. Simpson
通讯作者：
Ying Zhao;V. Agarwal;C. Mendelson;E. Simpson
DOI：
10.1210/jcem.81.1.8550748
发表时间：
1996
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
L. Noble;E. Simpson;A. Johns;S. Bulun
通讯作者：
L. Noble;E. Simpson;A. Johns;S. Bulun
DOI：
10.1152/ajpendo.1981.241.6.e444
发表时间：
1981-12
期刊：
The American journal of physiology
影响因子：
--
作者：
R. Sridaran;G. Gibori
通讯作者：
R. Sridaran;G. Gibori
影响因子：
--
作者：
I. GONZALEZ-ROBAYNA;Tamara Alliston;Patricia Buse;G. Firestone;J. S. Richards
通讯作者：
I. GONZALEZ-ROBAYNA;Tamara Alliston;Patricia Buse;G. Firestone;J. S. Richards
影响因子：
4.8
作者：
A. Nackley;W. Shea-Eaton;D. Lopez;M. McLean
通讯作者：
A. Nackley;W. Shea-Eaton;D. Lopez;M. McLean