Electron microscopic and preparative methods for the analysis of isopod cuticle.
Electron microscopic and preparative methods for the analysis of isopod cuticle.
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电子显微镜和制备方法，用于分析异脚类角质层。
DOI：
10.3897/zookeys.176.2294
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发表时间：
2012
期刊：
影响因子：
1.3
通讯作者：
Ziegler A
中科院分区：
文献类型：
--
作者：
Seidl BH;Ziegler A
甲壳动物角质层由复杂的有机基质和矿物相组成。角质层的物理和化学性质与角质层元素的特定功能相关，导致其结构和成分的多样性。甲壳类动物角质层结构与功能关系的研究需要复杂的方法工具来分析角质层结构的不同方面。在本文中，我们报告了改进的制备方法，结合各种电子显微镜技术，对 Porcellio scaber 的 tergite 角质层的角质层结构和成分有了新的认识。我们使用非脱钙土石和脱钙树脂包埋材料的薄片进行透射电子显微镜和扫描透射电子显微镜。用场发射扫描电子显微镜分析了块状白镁矿样品的蚀刻矢状面。我们发现外表皮内有一个明显的远端区域，其纤维排列不同于邻近的近端外表皮。在该远端外表皮内，几丁质蛋白原纤维组装成直径在 15 至 50 nm 之间的纤维，嵌入矿物基质中。在近端外表皮和内表皮中，原纤维不聚集成纤维，并且单独地被矿物质包围。此外，我们表明孔道充满了矿物质，并证明抛光矢状角质层表面的轻微蚀刻揭示了含有不同溶解度矿物质的区域。
The crustacean cuticle consists of a complex organic matrix and a mineral phase. The physical and chemical properties of the cuticle are corellated to the specific functions of cuticular elements, leading to a large variety in its structure and composition. Investigation of the structure-function relationship in crustacean cuticle requires sophisticated methodological tools for the analysis of different aspects of the cuticular architecture. In the present paper we report improved preparation methods that, in combination with various electron microscopic techniques, have led to new insights of cuticle structure and composition in the tergite cuticle of Porcellio scaber. We used thin sections of non-decalcified tergites and decalcified resin embedded material for transmission electron microscopy and scanning transmission electron microscopy. Etched sagittal planes of bulk tergite samples were analysed with field emission scanning electron microscopy. We have found a distinct distal region within the exocuticle that differs from the subjacent proximal exocuticle in the arrangement of fibres. Within this distal exocuticle chitin-protein fibrils assemble to fibres with diameters between 15 and 50 nm that are embedded in a mineral matrix. In the proximal exocuticle and the endocuticle fibrils do not assemble to fibres and are surrounded by mineral individually. Furthermore, we show that the pore canals are filled with mineral, and demonstrate that mild etching of polished sagittal cuticle surfaces reveals regions containing mineral of diverse solubility.
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影响因子：
3
作者：
Hild, Sabine;Marti, Othmar;Ziegler, Andreas
通讯作者：
Ziegler, Andreas
DOI：
10.1007/bf00998125
发表时间：
1980-01-01
期刊：
ZOOMORPHOLOGIE
影响因子：
--
作者：
PRICE, JB;HOLDICH, DM
通讯作者：
HOLDICH, DM
α-几丁质（聚-N-乙酰基-D-葡萄糖胺）的晶体结构。
DOI：
10.1083/jcb.3.5.669
发表时间：
1957-09-25
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
CARLSTROM, D
通讯作者：
CARLSTROM, D
影响因子：
2.6
作者：
GIRAUDGUILLE, MM
通讯作者：
GIRAUDGUILLE, MM
影响因子：
3.5
作者：
Bobelmann, F.;Romano, P.;Epple, M.
通讯作者：
Epple, M.