Comprehensive silk gland multi-omics comparison illuminates two alternative mechanisms in silkworm heterosis.
Comprehensive silk gland multi-omics comparison illuminates two alternative mechanisms in silkworm heterosis.
复制标题
全面的丝腺多组学比较阐明了家蚕杂种优势的两种替代机制
DOI：
10.24272/j.issn.2095-8137.2022.065
复制
发表时间：
2022-07-18
影响因子：
4.9
通讯作者：
中科院分区：
文献类型：
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作者：
杂种优势是植物和动物中普遍存在的现象，其潜在机制多种多样。本研究采用两种广泛应用的家蚕杂交系统，通过多组学分析，确定不同杂交策略和表观遗传机制与家蚕杂种优势之间可能存在的内在联系。我们发现两种系统的蚕丝腺转录组景观存在显著差异，包括杂交后代与亲本相比的差异表达基因和表达模式。在四元杂交体系中，杂种优势主要源于基因上调和亲本显性表达模式的上调，涉及多种转运过程、细胞氮化合物分解代谢、葡萄糖代谢和三羧酸循环。在二元体系中，杂种优势主要是由于基因下调和表达模式越界下调，主要涉及基本氮合成代谢和机体功能。我们还证明了DNA甲基化可能通过调节几个杂种优势相关基因的表达来影响杂种活力。因此，本研究揭示了两种可能有助于家蚕杂种优势的机制，这两种机制都有助于有效利用能量和氮来生产蚕丝。
Heterosis is a common phenomenon in plants and animals with diverse underlying mechanisms. Here, we applied two widely used silkworm hybrid systems and performed multi-omics analysis to identify possible intrinsic associations between different hybrid strategies and epigenetic mechanisms with silkworm heterosis. We found significant differences in the silk gland transcriptomic landscape between the two systems, including differentially expressed genes and expression patterns in the hybrid offspring compared to their parents. In the quaternary hybrid system, hybrid vigor was primarily due to up-regulated genes and the parent-dominant up-regulated expression pattern, involving multiple transport processes, cellular nitrogen compound catabolism, glucose metabolism, and tricarboxylic acid cycle. In the binary system, hybrid vigor was mainly due to the down-regulated genes and transgressively down-regulated expression pattern, mainly involving basic nitrogen synthesis metabolism and body function. We also demonstrated that DNA methylation may affect hybrid vigor by regulating the expression of several heterosis-related genes. Thus, this study revealed two alternative mechanisms that may contribute to silkworm heterosis, both of which facilitate the efficient utilization of energy and nitrogen for silk production.
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