The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation.
The Natural Cytotoxicity Receptor NKp44 (NCR2, CD336) Is Expressed on the Majority of Porcine NK Cells Ex Vivo Without Stimulation.
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天然的细胞毒性受体NKP44（NCR2，CD336）在大多数猪NK细胞中表达而没有刺激。
DOI：
10.3389/fimmu.2022.767530
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发表时间：
2022
影响因子：
7.3
通讯作者：
Lunney JK
中科院分区：
文献类型：
--
作者：
Mair KH;Crossman AJ;Wagner B;Babasyan S;Noronha L;Boyd P;Zarlenga D;Stadler M;van Dongen KA;Gerner W;Saalmüller A;Lunney JK
自然杀伤(NK)细胞因其在脊椎动物先天免疫系统中的重要作用而在人类和小鼠中得到了广泛的研究。众所周知，它们可以利用其裂解特性在免疫反应中迅速消除肿瘤或病毒感染的细胞。天然细胞毒受体NKp30(NCR3)、NKp44(NCR2)和NKp46(NCR1)是NK细胞杀伤的重要介导物。据报道，NKp44在人类和一些非人类灵长类动物的NK细胞中都有表达，并且仅在激活的NK细胞上发现。此前，由于缺乏物种特异性的单抗，目前还没有关于NKp44蛋白在猪淋巴细胞中表达及其作用的信息。本研究制备了猪特异性抗NKp44单抗，并检测了其与不同日龄猪血液和组织来源的NK细胞的反应性。有趣的是，在体外已经检测到NKp44在静息NK细胞上的表达；而且，在所分析的大多数动物中，NKp44+NK细胞的频率高于NKp46+NK细胞。经IL-2或IL-15体外刺激后，NKp44+NK细胞比例增加，NKp44在单细胞水平的表达强度增加。由于对猪NK细胞知之甚少，针对NKp44的mAb(克隆54-1)的制备将有助于阐明猪NK细胞分化、功能和激活的机制。
Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural cytotoxicity receptors (NCRs) NKp30 (NCR3), NKp44 (NCR2), and NKp46 (NCR1) are important mediators of NK-cell cytotoxicity. NKp44 expression was reported for NK cells in humans as well as in some non-human primates and found exclusively on activated NK cells. Previously, no information was available on NKp44 protein expression and its role in porcine lymphocytes due to the lack of species-specific monoclonal antibodies (mAbs). For this study, porcine-specific anti-NKp44 mAbs were generated and their reactivity was tested on blood and tissue derived NK cells in pigs of different age classes. Interestingly, NKp44 expression was detected ex vivo already on resting NK cells; moreover, the frequency of NKp44+ NK cells was higher than that of NKp46+ NK cells in most animals analyzed. Upon in vitro stimulation with IL-2 or IL-15, the frequency of NKp44+ NK cells, as well as the intensity of NKp44 expression at the single cell level, were increased. Since little is known about swine NK cells, the generation of a mAb (clone 54-1) against NKp44 will greatly aid in elucidating the mechanisms underlying the differentiation, functionality, and activation of porcine NK cells.
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影响因子：
4.6
作者：
Hromadnikova I;Pirkova P;Sedlackova L
通讯作者：
Sedlackova L
影响因子：
64.8
作者：
Cella, Marina;Fuchs, Anja;Vermi, William;Facchetti, Fabio;Otero, Karel;Lennerz, Jochen K. M.;Doherty, Jason M.;Mills, Jason C.;Colonna, Marco
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作者：
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通讯作者：
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5.4
作者：
Mair, Kerstin H.;Essler, Sabine E.;Gerner, Wilhelm
通讯作者：
Gerner, Wilhelm
影响因子：
4.4
作者：
De Maria, Andrea;Ugolotti, Elisabetta;Biassoni, Roberto
通讯作者：
Biassoni, Roberto